Polyethylenimines

Interfdci l Composite Membra.nes, A method of making asymmetric membranes involving interfacial polymerization was developed in the 1960s. This technique was used to produce reverse osmosis membranes with dramatically improved salt rejections and water fluxes compared to those prepared by the Loeb-Sourirajan process (28). In the interfacial polymerization method, an aqueous solution of a reactive prepolymer, such as polyamine, is first deposited in the pores of a microporous support membrane, typically a polysulfone ultrafUtration membrane. The amine-loaded support is then immersed in a water-immiscible solvent solution containing a reactant, for example, a diacid chloride in hexane. The amine and acid chloride then react at the interface of the two solutions to form a densely cross-linked, extremely thin membrane layer. This preparation method is shown schematically in Figure 15. The first membrane made was based on polyethylenimine cross-linked with toluene-2,4-diisocyanate (28). The process was later refined at FilmTec Corporation (29,30) and at UOP (31) in the United States, and at Nitto (32) in Japan. [Pg.68]

The acid/base interaction between the two polymers significantly increases the cohesive strength of the polymer blend at normal use temperatures but at elevated temperature the interaction can be interrupted and the polymer can still be melt processed. Other examples of basic polymers use for crosslinking include polyethylenimines, vinyl pyridine copolymers, and the like. [Pg.499]

UPEI), and poly[l-(uridin-5 -yl)-iminoethylene halide], 13 (UPEI ). They were obtained by quaternization of poly-4-vinylpyridine or polyethylenimine with ehlo-roethylated, ehloropropylated, or ehlorated nucleic acid bases. [Pg.140]

Klotz etal.U9, l2° asserted that polymers from polyethylenimine reacted with chloromethylimidazole and dodecyliodide were excellent models for hydrolase. 70 [PEI-D(10%)-Im(l 5%)] catalyzed the hydrolyses of PNPA 270 times faster than imidazole itself. The authors also found that lauroylated polyethylenimine containing hydroxamate and imidazole groups, 71 [PEI-HA(8%)-L(8%)-Im(6.6%)], had a high efficiency in hydrolyses, i. e. 310 times that of imidazole. [Pg.164]

Primary amino- and SH-groups are also nucleophiles and active for hydrolyses. Klotz et al.12S 127) investigated the activities of lauroyl-substituted polyethylenimine,... [Pg.165]

Kabanov et aLl2S studied the hydrolyses of p-nitrophenylesters in the presence of linear and branched benzylated polyethylenimine, 76 (BzPEI). The catalytic site... [Pg.166]

The action of polyethylenimine on the hydrolyses of 43 (aspirin) was characteristic95 the reaction was substantially increased, passing through a maximum at pH = 7.8, where the rate constant was 1300 times greater than in the absence of the polyelectrolyte. [Pg.167]

The hydrolyses of ADP and ATP were tried in the presence of low-molecular-weight analogs of polyethylenimine, i e. ethylenediamine, 77 (ED), diethylenetriamine, 78 (DT), triethylenetetramine, 79 (TT), tetraethylenepentamine, 80 (TP) and pentaethylenehexamine129). These compounds accelerated the reaction several times. The degree of the acceleration increased with increasing degree of polymerization. [Pg.167]

A thermally stable NHase from Comamonas testosteroni 5-MGAM-4D (ATCC 55 744) [22] was recombinantly expressed in Escherichia coli, and the resulting transformant cells immobilized in alginate beads that were subsequently chemically cross-linked with glutaraldehyde and polyethylenimine. This immobilized cell catalyst (at 0.5 % dew per reaction volume) was added to an aqueous reaction mixture containing 32wt% 3-cyanopyridine at 25 °C, and a quantitative conversion to nicotinamide was obtained. The versatility of this catalyst system was further illustrated by a systematic study of substrates, which included... [Pg.171]

S. S. Diebold, H. Lehrmann, M. Kursa, E. Wagner, M. Cotten, and M. Zenke, Efficient gene delivery into human dendritic cells by adenovirus polyethyleni-mine and mannose polyethylenimine transfection, Hum. Gene Ther., 10 (1999) 775-786. [Pg.386]

Boussif, O., Lezoualc h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., and Behr, J.P., A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo polyethylenimine, Proceedings of the National Academy of Sciences, United States of America, 1995, 92, 7297-7301. [Pg.15]

Aigner A, Fischer D, Merdan T, Bms C, Kissel T, Czubayko F (2002) Delivery of unmodified bioactive ribozymes by an RNA-stabilizing polyethylenimine (LMW-PEI) efficiently down-regulates gene expression. Gene Ther 9 1700-1707... [Pg.19]

Zou SM, Erbacher P, Remy JS, Behr JP (2000) Systemic linear polyethylenimine (L-PEI)-mediated gene delivery in the mouse. J Gene Med 2 128-134... [Pg.20]

Creusat G, Zuher G (2008) Self-assembling polyethylenimine derivatives mediate efficient siRNA delivery in mammalian cells. Chembiochem 9 2787-2789... [Pg.21]

Lemkine GF, Goula D, Becker N, Paleari L, Levi G, Demeneix BA (1999) Optimisation of polyethylenimine-based gene delivery to mouse brain. J Drug Target 7 305-312... [Pg.21]

Malek A, Czubayko F, Aigner A (2008) PEG grafting of polyethylenimine (PEI) exerts different effects on DNA transfection and siRNA-induced gene targeting efficacy. J Drug Target 16 124-139... [Pg.22]

Brus C, Petersen H, Aigner A, Czubayko F, Kissel T (2004) Physicochemical and biological characterization of polyethylenimine-graft-poly(ethylene glycol) block copolymers as a delivery system for oligonucleotides and ribozymes. Bioconjug Chem 15 677-684... [Pg.22]

Rudolph C, Schillinger U, Plank C, Gessner A, Nicklaus P, Muller R, Rosenecker J (2002) Nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine 1. Biochim Biophys Acta 1573 75... [Pg.22]

Carlisle RC, Etrych T, Briggs SS, Preece JA, Ulbrich K, Seymour LW (2004) Polymer-coated polyethylenimine/DNA complexes designed for triggered activation by intracellular reduction. J Gene Med 6 337-344... [Pg.22]

Kurosaki T, Kitahara T, Fumoto S, Nishida K, Nakamura J, Niidome T, Kodama Y, Nakagawa H, To H, Sasaki H (2009) Ternary complexes of pDNA, polyethylenimine, and gamma-polyglutamic acid for gene delivery systems. Biomaterials 30 2846-2853... [Pg.23]

Kircheis R, Wightman L, Schreiber A, Robitza B, Rossler V, Kursa M, Wagner E (2001) Polyethylenimine/DNA complexes shielded by transferrin target gene expression to tumors after systemic application. Gene Ther 8 28 10... [Pg.23]

Pons B, Mouhoubi L, Adib A, Godzina P, Behr JP, Zuber G (2006) omega-Hydrazino linear polyethylenimine a monoconjugation building block for nucleic acid delivery. Chembiochem 7 303-309... [Pg.23]

Chiu SJ, Ueno NT, Lee RJ (2004) Tumor-targeted gene delivery via anti-HER2 antibody (trastuzumab, Herceptin) conjugated polyethylenimine. J Control Release 97 357-369... [Pg.24]

Moffatt S, Wiehle S, Cristiano RJ (2005) Tumor-specific gene delivery mediated by a novel peptide-polyethylenimine-DNA polyplex targeting aminopeptidase N/CD13. Hum Gene Ther 16 57-67... [Pg.25]

Elfinger M, Pfeifer C, Uezguen S, Golas MM, Sander B, Maucksch C, Stark H, Aneja MK, Rudolph C (2009) Self-assembly of ternary insulin-polyethylenimine (PEI)-DNA nanoparticles for enhanced gene delivery and expression in alveolar epithelial cells. Biomacromolecules 10 2912-2920... [Pg.26]

Gabrielson NP, Pack DW (2009) Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells. J Control Release 136 54—61... [Pg.26]

Oskuee RK, Philipp A, Dehshahri A, Wagner E, Ramezani M (2010) The impact of carboxyalkylation of branched polyethylenimine on effectiveness in small interfering RNA delivery. J Gene Med 12 729-738... [Pg.26]

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