Polyethylenimine cells

A thermally stable NHase from Comamonas testosteroni 5-MGAM-4D (ATCC 55 744) [22] was recombinantly expressed in Escherichia coli, and the resulting transformant cells immobilized in alginate beads that were subsequently chemically cross-linked with glutaraldehyde and polyethylenimine. This immobilized cell catalyst (at 0.5 % dew per reaction volume) was added to an aqueous reaction mixture containing 32wt% 3-cyanopyridine at 25 °C, and a quantitative conversion to nicotinamide was obtained. The versatility of this catalyst system was further illustrated by a systematic study of substrates, which included... 

S. S. Diebold, H. Lehrmann, M. Kursa, E. Wagner, M. Cotten, and M. Zenke, Efficient gene delivery into human dendritic cells by adenovirus polyethyleni-mine and mannose polyethylenimine transfection, Hum. Gene Ther., 10 (1999) 775-786. 

Boussif, O., Lezoualc h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., and Behr, J.P., A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo polyethylenimine, Proceedings of the National Academy of Sciences, United States of America, 1995, 92, 7297-7301. 

Creusat G, Zuher G (2008) Self-assembling polyethylenimine derivatives mediate efficient siRNA delivery in mammalian cells. Chembiochem 9 2787-2789... 

Elfinger M, Pfeifer C, Uezguen S, Golas MM, Sander B, Maucksch C, Stark H, Aneja MK, Rudolph C (2009) Self-assembly of ternary insulin-polyethylenimine (PEI)-DNA nanoparticles for enhanced gene delivery and expression in alveolar epithelial cells. Biomacromolecules 10 2912-2920... 

Gabrielson NP, Pack DW (2009) Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells. J Control Release 136 54—61... 

BrunnerS, FurtbauerE, Sauer T, KursaM, Wagner E (2002) Overcoming the nuclear barrier cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation. Mol Ther 5 80-86... 

Itaka K, Harada A, Yamasaki Y, Nakamura K, Kawaguchi H, Kataoka K (2004) In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine. J Gene Med 6 76-84... 

Wiseman, J.W., Goddard, C.A., McLelland, D., Colledge, W.H. (2003). A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo. Gene Then, 10(19), 1654—1662. 

Diebold, S.S., Kursa, M., Wagner, E., Cotten, M. andZenke,M. (1999) Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells. J. Biol. Client., 274, 18087-19094. 

Pollard, H., Remy, J.S., Loussouam, G., Demolombe, S., Behr, J.P. and Escande, D. (1998) Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J. Biol. Chem., 273,7507-7511. 

In an original manner, one SV40 NLS sequence has been covalently linked to one end of a linear plasmid encoding Luciferase (Zanta et al., 1999). In combination with polyethylenimine, transfection efficiency was increased 10- to 1000-fold, depending on the cell types compared to the same construction containing a nonfunctional mutated NLS. 

As for polyethylenimines and polyamidoamines, PDMAEMA has been assessed for its transfection ability in vivo. Van de Wetering et al. (1999b) examined the efficiency of PDMAEMA in vivo and found that while ovarian carcinoma cells were able to be transfected in vitro and ex vivo at efficiencies up to 10%, they were not transfected in vivo. Components present in body fluids that are not present in the in vitro model may be responsible for the disappearance of transfection activity. This idea is supported by evidence that the presence of hyaluronic acid, one of many fluid components, greatly reduced in vitro transfection efficiency. 

Marschall, P., Malik, N. and Larin, Z. (1999) Transfer of YAC s up to 2.3Mb intact into human cells with polyethylenimine. Gene Ther., 6, 1634-1637. 

Poulain, L., Ziller, C., Muller, C.D., Erbacher, P., Bettinger, T., Rodier, J.-F. and Behr, J.-P. (2000) Ovarian carcinoma cells are effectively transfected by polyethylenimine (PEI) derivatives. Cancer Gene Ther., 7, 644-652. 

Wojda, U. and Miller, J.L. (2000) Targeted transfer of polyethylenimine-avidin-DNA bioconjugates to hematopoietic cells using biotinylated monoclonal antibodies. J. Pharm. Sci., 89, 674-681. 

Abstract Carbohydrates have been investigated and developed as delivery vehicles for shuttling nucleic acids into cells. In this review, we present the state of the art in carbohydrate-based polymeric vehicles for nucleic acid delivery, with the focus on the recent successes in preclinical models, both in vitro and in vivo. Polymeric scaffolds based on the natural polysaccharides chitosan, hyaluronan, pullulan, dextran, and schizophyllan each have unique properties and potential for modification, and these results are discussed with the focus on facile synthetic routes and favorable performance in biological systems. Many of these carbohydrates have been used to develop alternative types of biomaterials for nucleic acid delivery to typical polyplexes, and these novel materials are discussed. Also presented are polymeric vehicles that incorporate copolymerized carbohydrates into polymer backbones based on polyethylenimine and polylysine and their effect on transfection and biocompatibility. Unique scaffolds, such as clusters and polymers based on cyclodextrin (CD), are also discussed, with the focus on recent successes in vivo and in the clinic. These results are presented with the emphasis on the role of carbohydrate and charge on transfection. Use of carbohydrates as molecular recognition ligands for cell-type specific dehvery is also briefly... 

Jiang H-L, Kim Y-K, Arote R et al (2009) Mannosylated chitosan-graft-polyethylenimine as a gene carrier for Raw 264.7 cell targeting. Int J Pharm 375(1-2) 133—139... 

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