Target gene expression

Imamura, M., Nakai, J., Inoue, S., Quan, G. X., Kanda, T., and Tamura, T. 2003. Targeted gene expression using the GAL4/UAS system in the silkworm Bombyx mori. Genetics, 165(3) 1329-1340. 

Kircheis R, Wightman L, Schreiber A, Robitza B, Rossler V, Kursa M, Wagner E (2001) Polyethylenimine/DNA complexes shielded by transferrin target gene expression to tumors after systemic application. Gene Ther 8 28 10... 

Brand AH, Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118 401-415... 

Wu, G.Y., Zhan, P, Sze, L.L., Rosenberg, A.R., Wu, C.H. (1994). Incorporation of adenovirus into a ligand-based DNA carrier system results in retention of original receptor specificity and enhances targeted gene expression. J. Biol. Chem., 269, 11542-11546. 

LIMITATION OF PNA BINDING-INDUCED TARGET GENE EXPRESSION... 

Flannery, J. G., Zolotukhin, S., Vaquero, M. I., LaVail, M. M., Muzyczka, N. and Hauswirth, W. W. (1997). Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus. Proc. Natl. Acad. Sci. USA 94, 6916-6921. 

Add the transfection reagent/siRNA complex mixture dropwise to the cells. Gently rock the plate back and forth and from side-to-side to distribute the complexes evenly and incubate for 24-72 h. Finally, perform assay for inhibition of target gene expression as described in Subheading 3.4.5. 

Model for Vitamin D-influenced Target Gene Expression... 

Jesch, S.A., Zhao, X., Wells, M.T., and Henry, S.A., 2005, Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast. J. Biol. Chem. 280 9106-9118. 

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