Polyethylenimine protein

The other possibility is to coat the silica with a polymer of defined properties (molecular weight and distribntion) and olefin groups, e.g., polybutadiene, and cross-linked either by radiation or with a radical starter dissolved in the polymer [32]. This method is preferentially used when other carriers like titania and zirconia have to be surface modified. Polyethylenimine has been cross-linked at the snrface with pentaerythrolglycidether [41] to yield phases for protein and peptide chromatography. Polysiloxanes can be thermally bonded to the silica surface. Other technologies developed in coating fnsed silica capillaries in GC (polysiloxanes with SiH bonds) can also be applied to prepare RP for HPLC. 

Carlisle, R.C., Bettinger, T., Ogris, M., Hale, S., Mautner, V., Seymour, L.W. (2001). Adenovirus hexon protein enhances nuclear delivery and increases transgene expression of polyethylenimine/plasmid DNA vectors. Mol. Then, 4(5), 473 -83. 

Theoretical studies were reported of the thiolysis by 2-mercaptoethanol and 2-mer-captoethylamine of simple models of the bicyclic ring systems of penicillin and cephalosporin88 and of the alkaline hydrolysis of sanfetrinem (93)89 The major determinant in allergies induced by penicillins is the penicilloyl group bound to the amino group of the Lys residues present in the carrier protein. Now a study is reported of the polyelectrolyte polyethylenimine (94) as a model of the carrier protein and its catalysis of the aminolysis of benzylpenicillin.90... 

In an earlier investigation by the author [1] electrophoresis protein separation membranes were prepared using triacryloyl-tris(2-aminoethyl)amine, (I), tri-methacryloyl-tris(2-aminoethyl)amine, (II), and polyethylenimine acrylates, (III). These agents were polymerized either by using ammonium persulphate or photolytically. 

Table VIdesoibes the further derivatization ofapolyethylenimine-coated support, possessing an underivatized picric add exchange capacity of. a 280 Amol N/g support 40). Various monoepoxides containing a range of chemistries were bonded on to the polyethylenimine-support. As the chemical nature of the support changed relative retention of test proteins varied.

Polyethylenimine-coated supports have been used in the separation of proteins, nucleotides, and sinde>stranded oligonudeotides. These coated... 

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