Nucleic acids, delivery

Peptoids have also shown great utility in their ability to complex with and deliver nucleic acids to cells, a critical step toward the development of antisense drugs, DNA vaccines, or gene-based therapeutics. Most non-viral nucleic acid delivery systems are based on cationic molecules that can form complexes with the polyan-... 

Chinnery P.E., Taylor R.W., Diekert K., Lill R., Turnbull D.M., Lightow-LERS R.N. Peptide nucleic acid delivery to human mitochondria. Gene Therapy 1999 6 1919-1928. 

The potential of the chemically modified nucleic acid molecules has been proven by in vitro studies however, the in vivo therapeutic applicability of these molecules seems to be unsatisfactory because of their possible toxic effects (largely unknown) and adverse bioavailability. In this view, both antisense and transfection technologies require reliable and efficient systems for their delivery into target cells. On the basis of this consideration, the development of an efficient nucleic acid delivery system represents one of the key steps for these therapeutic agents, which are necessary for a practical clinical utilization of natural or unnatural oligonucleotides. 

Cortesi, R., Esposito, E., Magnan, M., Campi, F., and Nastruzzi, C., Cationic lipospheres for nucleic acid delivery, manuscript in preparation. 

Functional Polymer Conjugates for Medicinal Nucleic Acid Delivery... 

Pons B, Mouhoubi L, Adib A, Godzina P, Behr JP, Zuber G (2006) omega-Hydrazino linear polyethylenimine a monoconjugation building block for nucleic acid delivery. Chembiochem 7 303-309... 

Wagner E, Kircheis R, Walker GF (2004) Targeted nucleic acid delivery into tumors new avenues for cancer therapy. Biomed Pharmacother 58 152-161... 

Concerning utilisation of CNTs for the delivery of nucleic acids, CNTs have already been shown to be versatile platforms for nucleic acid delivery in vitro and in vivo because of their high surface area, facile functionalisation of their surface and their ability to cross the cell membranes. To our knowledge, it is crucial to functionalise the surface of CNTs, in order to transform nonfunctionalised CNTs (insoluble in most solvents) into water-soluble and biocompatible CNTs. On the other hand, with a growing number of functionalisation routes, many important questions remain unanswered. Each functionalisation method is probably producing... 

Pichon, C., C. Goncalves, and P. Midoux. 2001. Histidine-rich peptides and polymers for nucleic acids delivery. Adv Drug Deliv Rev 53(1) 75-94. 

Compositions and methods for nucleic acid delivery to the lung. Inhale Therapeutic Systems Inc., San Carlos, CA. U.S. Patent No. 5994314, issued 11-30-1999. 

Interstitial transport of macromolecules implication for nucleic acid delivery in solid tumors... 

In recent years cationic dendritic structures such as carbosilane-based [215], triazine-based [216], polyester [217] and amphiphilic dendrimers [218] have been investigated for transfection purposes with promising results, thus offering novel alternatives to the exploration of dendrimer-based nucleic acid delivery. 

Abstract Carbohydrates have been investigated and developed as delivery vehicles for shuttling nucleic acids into cells. In this review, we present the state of the art in carbohydrate-based polymeric vehicles for nucleic acid delivery, with the focus on the recent successes in preclinical models, both in vitro and in vivo. Polymeric scaffolds based on the natural polysaccharides chitosan, hyaluronan, pullulan, dextran, and schizophyllan each have unique properties and potential for modification, and these results are discussed with the focus on facile synthetic routes and favorable performance in biological systems. Many of these carbohydrates have been used to develop alternative types of biomaterials for nucleic acid delivery to typical polyplexes, and these novel materials are discussed. Also presented are polymeric vehicles that incorporate copolymerized carbohydrates into polymer backbones based on polyethylenimine and polylysine and their effect on transfection and biocompatibility. Unique scaffolds, such as clusters and polymers based on cyclodextrin (CD), are also discussed, with the focus on recent successes in vivo and in the clinic. These results are presented with the emphasis on the role of carbohydrate and charge on transfection. Use of carbohydrates as molecular recognition ligands for cell-type specific dehvery is also briefly... 

Keywords Carbohydrate DNA Nucleic acid delivery polysaccharide Transfection... 

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