P-388 murine lymphocytic leukemia

In addition to silymarin, hydnocarpin (68) and sinaiticin (96) isolated from Verbascum sinaiticum exhibited cytotoxicity against cultured P-388 (murine lymphocytic leukemia) cells (120). 5 -Methoxyhydnocarpin-D (79) and silybin (1) have been identified as bacterial MDR pump inhibitors (107). 

Berberine chloride was demonstrated to possess significant cytotoxic activity against cultured P-388 (murine lymphocytic leukemia) cells, in addition to three human cancer cell lines BC1 (breast cancer) - EDJ0 2.7 pM) HT-1080 (fibrosarcoma) - ED50 2.4 pM) and KB (nasopharyngeal carcinoma) - EDJ0 8.4 pM)[336]. 

Berberrubine chloride was found to exhibit cytotoxic activity against cultured P-388 (murine lymphocytic leukemia) cells (1.7 pM)[336]. 

Podophyllotoxin (1), 4 -danethyldeoxypodophyUotoxin (2) and P-apopicro-podophyllin (3) strongly inhibited P-388 (murine lymphocytic leukemia) the IC50 values (concentration at which 50% of cells were killed) were 0.003, 0.005, and... 

The bioactivity data for Pseudobersama sterols are given in Table 6. All three sterols exhibited selective activity on the rad52 strain compared with the wild-type RAD+ strain. Ergost-5,24(28)-diene-3p,7a-diol (37) was also tested in P-388 murine lymphocytic leukemia assay and was found to be moderately cytotoxic. 

We have isolated two 3,7-dihydroxysteroidal compounds from S. glutinosa, one known 7-a-hydroxysitosterol and one new l-oxo-7a-hydroxysitosterol (70). According to the literature , cholest-5-ene-3p,7P-diol derivatives showed activity in the P-388 murine lymphocytic leukemia test... 

Ansamycin antibiotics are probably the most complex organic compounds produced by the genus Streptomyces. In addition to the antibacterial, they also exhibit antiviral effects. Ansamitocin P-3 shows a potent cytotoxicity against the human solid tumor cell lines A-549 and HT-29. The compound exhibits a significant activity against P-388 lymphocytic leukemia in mice and both 9PS (murine lymphocytic leukemia) and 9KB (human nasopharyngeal carcinoma) in cell culture systems. [38]. 

Cytotoxicity is denoted by inhibition of human epidermal carcinoma (KB-16), human lung adenocarcinoma (A549), human colon carcinoma (HT-29), and murine lymphocytic leukemia (P-388) cells at half maximal inhibitory concentration (IC50) values of pg/mL. bFor the cell lines tested, an IC50 value of <4 pg/mL is regarded as active. 

Bruceanols D (67), E (68), and F (69) were tested for in vitro cytotoxicity in five human tumor cell lines (KB, A-549, HCT-8, RPMI-7951, TE-671) and a murine lymphocytic leukemia (P-388). The results shown in Table 1 [37], All the compounds showed significant activity in each tested cell lines. 

Gris (58). 13,18-Dehydroglaucarubinone (36b) was found in Simarouba amara (91) and in Ailanthus altissima (36), 13,18-dehydroailanthinone (37b) in Pierrodendron kerstingii (46) and 13,18-dehydroexcelsin (38) was found in Ailanthus excelsa (40) and in A.malabarica (39). Only those quassinoids which possess an a, 3-unsaturated ketol group in ring A display significant antineoplastic activity in the NIH murine lymphocytic leukemia P 388 (PS) system. 

Pancratistatin (15) (Figure 7.4) was isolated by Pettit and co-workers from the roots of Pancratium littorale, which exhibits anticancer activity against murine P-5076 ovarian sarcoma and P-388 lymphocytic leukemia [10]. Rigby and coworkers performed photocycHzation as a key step in the total synthesis of 15,... 

Daphneticin (12), isolated from Daphne tangutica, showed cytotoxic activity against Walker carcinosarcoma ascites cells (68) but was inactive against KB cells (68). In vitro studies showed cleomiscosin A (9), isolated from the chloroform extract of Brucea javanica, to be active against the murine P-388 lymphocytic leukemia cell line (EDsq = 0.4 pg ml ), but inactive against the KB test system (57). Luyengi et al. reported the lack of activity of the same compound when evaluated against HL-60 human promyelocytic leukemia cells (59). 

Yadanziosides F (93), I (94), J (95), and L (96) were demonstrated to have in vivo antileukemic activity against the murine P-388 lymphocytic leukemia. 

Bruceanol C (66) demonstrated potent cytotoxicity against human KB, A-549 lung carcinoma, and HCT-8 colon tumor as well as murine P-388 lymphocytic leukemia with ED o values of < 0.04, 0.48, <0.40, and 0.56 pg/mL, respectively [36],... 

Cytotoxicity by palytoxins has also been demonstrated in human bronchial epithelial cells [69], 3T3 Swiss mouse cells [70], V79 Chinese hamster lung cells [71], H 2981 human lung adenocarcinoma cells [72], EL-4 murine T-lymphoma cells [72], HL-60 human promyelocytic leukemia cells [73], and by an extract of O. siamensis in neuroblastoma cells [19], Palytoxin was shown to be highly toxic to P-388 lymphocytic leukemia cells [15]. Ostreocin D was significantly less toxic than palytoxin to the last-named cells [15]. Palytoxin, at very low concentration, inhibited the motility... 

Other lignans with known antitumor properties are (+)-wikstromol 90 isolated from Wikstroemia foetida var. oahuensis Gray (Thymelaeaceae) (92) and (-)-burseran 91 from Bur sera microphylla (Burseraceae) (93). (-)-Steganacin 92 and (-)-steganangin 93, isolated from Steganotaenia araliacea stem bark and wood, exhibit antileukemic activity in the in vivo murine P-388 lymphocytic leukemia test system (43). 

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