Bronchial epithelial cells, human

Hewson CA, Jardine A. Edwards MR. Laza-Stanca V, Johnston SL Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells. J Virol 2005 79 12273-12279. 

GERWIN B J, SPILLARE E, FORRESTER K, LEHMAN T A, KISPERT J, WELSH J A, PFEIFER A M, LECHNER J F, BAKER s J, VOGELSTEIN B et al. (1992) Mutant p53 can induce tumorigenic conversion of human bronchial epithelial cells and reduce their responsiveness to a negative growth factor, transforming growth factor beta 1 , Proc Natl Acad Sci USA, 89, 2759-63. 

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Prakash, P., C. Liu, K. Q. Hu et al. 2004. Beta-carotene and beta-apo-14 -carotenoic acid prevent the reduction of retinoic acid receptor beta in benzo[a]pyrene-treated normal human bronchial epithelial cells. J Nutr 134(3) 667-673. 

Santy, A., Dziejman, M., Taha, R.A, Iarossi, AS., Neote, K., Garcia-Zepeda, E.A., Hamid, Q. and Luster, A.D. (1999) The T cell specific CXC chemokines IP-10, Mig, and I-TAC are expressed by activated human bronchial epithelial cells. Journal of Immunology 162, 3549-3558. 

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NHBE Normal human bronchial epithelial cells... 

Calu-3 (American type culture collection ATCC HTB-55) is a human bronchial epithelial cell line derived from an adenocarcinoma of the lung [59], This cell line has been shown to exhibit serous cell properties and form confluent monolayers of mixed cell phenotypes, including ciliated and secretory cell types [60], but the cilia are formed very irregularly and seem to disappear with increasing passage number (unpublished observations, C.E. and B.F.). Calu-3 cells have shown utility as a model to examine transport [61-63] and metabolism in human bronchial epithelial cells for many therapeutic compounds [64], Furthermore, they have been used in a number of particlecell interaction studies [65-67], The interactions between respiratory epithelial cells and particulates are discussed more in detail in Chap. 19. 

A relatively new cell line that has not to date been characterised for its use in biopharmaceutics is based on primary airway epithelial cells infected with retroviruses expressing hTERT and HPV-16 E6/E7 (NuLi-1) [54], NuLi-1 cells were cultured on plastic up to passage 30. When grown on collagen-coated, semi-permeable membranes (Millicell-PCF), NuLi-1 TEER decreased only slightly over the 30 passages from 685 31 to 389 21 ohm.cm2. The TEER of NuLi-1 is similar to that observed with the primary bronchial cultures of 532 147 ohm.cm2. Thus, NuLi-1 cells can form an electrically tight airway epithelial barrier that mimics active and passive ion transport properties of primary human bronchial epithelial cells [54],... 

Masui T, Wakefield LM, Lechner JF, LaVeck MA, Spom MB, Harris CC (1986) Type beta transforming growth factor is the primary differentiation-inducing serum factor for normal human bronchial epithelial cells. Proc Natl Acad Sci USA 83(8) 2438-2442. 

Galietta LJ, Lantero S, Gazzolo A, Sacco O, Romano L, Rossi GA, Zegarra-Moran O (1998) An improved method to obtain highly differentiated monolayers of human bronchial epithelial cells. In Vitro Cell Dev Biol Anim 34(6) 478-481. 

Mathias NR, Timoszyk J, Stetsko PI, Megill JR, Smith RL, Wall DA (2002) Permeability characteristics of calu-3 human bronchial epithelial cells In vitro-in vivo correlation to predict lung absorption in rats. J Drug Target 10(1) 31—40. 

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Ehrhardt C, Kneuer C, Laue M, Schaefer UF, Kim KJ, Lehr CM (2003) 16HBE14o- human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein, and caveolin-1. Pharm Res 20(4) 545-551. 

Steerenberg PA, Zonnenberg JA, Dormans JA, Joon PN, Wouters IM, van Bree L, Scheepers PT, Van Loveren H (1998) Diesel exhaust particles induced release of interleukin 6 and 8 by (primed) human bronchial epithelial cells (BEAS 2B) in vitro. Exp Lung Res 24(1 ) 85—100. 

Proud D, Subauste MC, Ward PE (1994) Glucocorticoids do not alter peptidase expression on a human bronchial epithelial cell line. Am J Respir Cell Mol Biol 11(1) 57—65. 

Ehrhardt C, Collnot EM, Baldes C, Becker U, Laue M, Kim KJ, Lehr CM (2006) Towards an in vitro model of cystic fibrosis small airway epithelium Characterisation of the human bronchial epithelial cell line CFBE41o-. Cell Tissue Res 323(3) 405-415. 

X, Gandia K, Vaughan MB, Wright WE, Gazdar AF, Shay JW, Minna JD (2004) Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins. Cancer Res 64(24) 9027-9034. 

Reddel RR, Salghetti SE, Willey JC, Ohnuki Y, Ke Y, Gerwin BI, Lechner JF, Harris CC (1993) Development of tumorigenicity in simian virus 40-immortalized human bronchial epithelial cell lines. Cancer Res 53(5) 985-991. 

Crawford EL, Weaver DA, DeMuth JP, Jackson CM, Khuder SA, Frampton MW, Utell MJ, Thilly WG, Willey JC (1998) Measurement of cytochrome P450 2A6 and 2E1 gene expression in primary human bronchial epithelial cells. Carcinogenesis 19(10) 1867—1871. 

Kuzuya Y, Adachi T, Hara H, Anan A, Izuhara K, Nagai H (2004) Induction of drug-metabolizing enzymes and transporters in human bronchial epithelial cells by beclomethasone dipropionate. IUBMB Life 56(6) 355-359. 

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Borchard G, Cassara ML, Roemele PE, Florea BI, Junginger HE (2002) Transport and local metabolism of budesonide and fluticasone propionate in a human bronchial epithelial cell line (Calu-3). J Pharm Sci 91(6) 1561—1567. 

Vaughan, M. B., R. D. Ramirez, W. E. Wright, J. D. Minna, and J. W. Shay. 2006. A three-dimensional model of differentiation of immortalized human bronchial epithelial cells. Differentiation 74(4) 141-8. 

Lechner, J. F., and M. A. LaVeck. 1985. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J Tissue Culture Methods 9 43-8. 

Brash, D. E., R. R. Reddel, M. Quanrud, K. Yang, M. P. Farrell, and C. C. Harris. 1987. Strontium phosphate transfection of human cells in primary culture Stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells. Mol Cell Biol 7(5) 2031-4. 

Coursen, J. D., W. P. Bennett, L. Gollahon, J. W. Shay, and C. C. Harris. 1997. Genomic instability and telomerase activity in human bronchial epithelial cells during immortalization by human papillomavirus-16 E6 and E7 genes. Exp Cell Res 235( 1 ) 245-53. 

F. Trump, and C. C. Harris. 1991. Human bronchial epithelial cells transformed by the c-raf-1 and c-myc protooncogenes induce multidifferentiated carcinomas in nude mice A model for lung carcinogenesis. Cancer Res 51 3793-3801. 

Lechner, J. F., A. Haugen, I. A. McClendon, and A. M. Shamsuddin. 1984. Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum. Differentiation 25 229-237. 

Czarlewski W, Campbell AM, Bousquet J, Davies RJ Effect of loratadine on nitrogen dioxide-induced changes in electrical resistance and release of inflammatory mediators from cultured human bronchial epithelial cells. J AUergy Clin Immunol 1999 104 93-99. 

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