Human colon carcinoma

OITATE M, NAKAKI R, KOYABU N, TAKANAGA H, MATSUO H, OHTANI H, SAWADA Y (2001) TranSCellular transport of genistein, a soybean-derived isoflavone, across human colon carcinoma cell line (Caco-2). Biopharm Drug Dispos. 22 23-9. 

In culture, the human colon carcinoma cell hne Caco-2 spontaneously differentiates at confluency into polarized cells with enterocyte-like characteristics. The principle of this approach consists of following the passage of the compound of interest from the apical or lumen-like sides to the basolateral or lymph-hke sides of Caco-2 cells, thus following the absorption of the compound per se. One obhgate step for fat-soluble nutrients such as carotenoids to cross the intestinal barrier is their incorporation into CMs assembled in the enterocytes. Under normal cell culture conditions, Caco-2 cells are unable to form CMs. When supplemented with taurocholate and oleic acid, Caco-2 cells were reported to assemble and secrete CMs. ... 

Inhibition of DNA topo I activity in HCT 116 (human colon carcinoma) cells by CPT, CPT-11, SN-38, TPT, and EGCG (Table 8) ... 

The above three equations (Eqs. 10-12) for the different cell lines are very similar to each other, which suggests that the inhibition against DNA topo I is probably one of the most important antitumor mechanisms for these compounds (CPT, CPT-11, SN-38, TPT, and EGCG) against the three human colon carcinoma (HCT 116, VACO 241, and SW 480) cell lines. In these equations, the number of data points (four or five) is small, but the correlations are statistically significant. 

Li A, Varney ML, Singh RK. Constitutive expression of growth regulated oncogene (gro) in human colon carcinoma cells with different metastatic potential and its role in regulating their metastatic potential. Clin Exp Metastasis 2004 21 571-579. 

Hidalgo, I. J. Kato, A. Borchardt, R. T., Binding of epidermal growth factor by human colon carcinoma cell (Caco-2) monolayers, Biochem. Biophys. Res. Commun. 160, 317-324 (1989). 

The Caco-2 cell line was isolated from a human colon carcinoma, and has been characterized as one of the best in vitro models of intestinal epithelium. Indeed, in contrast to other intestinal cell lines, Caco-2 cells are able to constitute a homogenous monolayer and to spontaneously differentiate into polarized cells, highly similar to human mature enterocytes, after approximately 2 weeks of culture. Furthermore, the Caco-2 cells present microvillosities at the apical side and have a high transmembrane resistivity, which confirms the fact that the cells are confluent and link to one another via gap junctions. Finally, they can absorb different compounds, express many enzymes involved in intestinal metabolic pathways (Pinto et al. 1983, Musto et al. 1995, Salvini et al. 2002), and give reproducible in vitro results consistent with results obtained in in vivo studies (Artursson and Karlsson 1991). 

Pinto, M. et al. (1983). Enterocyte-like differentiation an polarizationof the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47 323-330. 

Das, S.K., Hashimoto, T., Shimizu, K., and Kanazawa, K. 2005. Fucoxanthin induces cell cycle arrest at GO/ Gl phase in human colon carcinoma cells through up-regulation of p21WAFl/Cipl. Biochim Biophys Acta 30 328-335. 

Hoffmann, R, Ji, H., Moritz, R. L., Connolly, L. M., Frecklington, D. F., Layton, M. J., Eddes, J. S., Simpson, R. J. (2001). Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon carcinoma cell line LIM 1215 a non two-dimensional gel electrophoresis-based proteome analysis strategy. Proteomics 1(7), 807. 

Hiribarren, A., Heyman, M., L Helgouac h, A. and Desjeux, J.F. (1993) Effect of cytokines on the epithelial function of the human colon carcinoma cell line HT29 cll9A. Gut 34, 616-620. 

Borchardt, R. T., Characterization of the human colon carcinoma cell line (Caco-2) as a model for intestinal epithelial permeability, Gastroenterology 1989, 96, 736-749. 

M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assman, P., Happen, K., Fogh, J., Zweibaum, A., Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture, Biol. Cell 1983, 47, 323-330. 

E., Brattain, M. G., Zweibaum, A., Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells a survey of twenty cell lines, Cancer Res. 1988, 48, 1936-1942. 

Brandsch, M., Miyamoto, Y., Ganapathy, V., Leibach, F. H., Expression and protein C-dependent regulation of peptide/H+ co-transport system in the Caco-2 human colon carcinoma cell line, Biochem. J. 1994, 299, 253-260. 

Grasset, E., Pinto, M., Dussaulx, E., Z WEI BAUM, A., Desjeux, J.-F., Epithelial properties of human colonic carcinoma cell line Caco-2 electrical parameters, Am. J. Physiol. 1984, 247, C260-C267. 

Cell line human colon carcinoma cells... 

Schuetz, J. D., Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinatefy up-regulate these proteins in human colon carcinoma cells, Mol. Pharmacol. 1996, 49, 311-318. 

Simpson RJ et al. Proteomic analysis of the human colon carcinoma cell line (LIM 1215] development of a membrane protein database. Electrophoresis 2000 21 1707-1732. 

Wang W, VanAlstyne PC, Irons KA, Chen S, Stewart JW and Birt DF. 2004. Individual and interactive effects of apigenin analogs on G2/M cell-cycle arrest in human colon carcinoma cell lines. Nutr Cancer 48(1) 106—114. 

HCT-116 human colon carcinoma (ATCC, Bethesda MD) cells were grown in McCoy s 5A) and were routinely subcultured twice weekly. Antiproliferative assay was performed by chemoluminescence assay based on quantification of ATP. Cells in their exponential phase of growth were treated at different times (lh or 24h) with different concentrations of edotecarin or SN-38. For post-treatment recovery studies, cells were washed with PBS and left in drug-free culture medium. Then, cell medium was collected to avoid any cell loss. Cells in monolayer were washed, detached with trypsin, and collected in the medium. Cells were counted in a Multisizer 3 Coulter Counter to measure the drug s effects on growth inhibition. Samples were fixed either... 

In contrast to P-gp and the MRP proteins, the breast cancer resistance protein (BCRP) contains six transmembrane domains and only one ATP-binding domain. It was first cloned from the breast cancer cell line MCF-7 selected in doxombicin, in the presence of the P-gp inhibitor verapamil. It is found in many human tissues, such as the placenta, small intestine, colon, and liver [133], It is localized to the apical membrane of epithelial cells of the small intestine and colon and to the bile canalicular membrane in the liver and is involved in reducing intestinal uptake, increasing hepatobiliary excretion, etc., leading to diminished oral bioavailability. cDNA sequences identical to BCRP and named MXR and ABCP, respectively, were independently isolated from human colon carcinoma cells and human placenta [134], BCRP requires... 

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