Normal cell lines

ICAT reagents can be used to compare two different samples by mass spec analysis. For instance, one cell population can be treated with a drug candidate, while another one remains untreated and acts as a control. Alternatively, one cell population can represent a disease state and the control population is the normal cell line. After cell lysis, the proteins in each... 

The toxicity is not only based on the amount or size of silica nanoparticles, but also on the cell line [95]. Cancer cell lines (A549, MKN-28) had a higher viability and resistance to silica nanoparticles than did normal cell lines (MRC-5, WS1 and CCD-966sk) [111]. Similarly, a previous study showed that A549 cells were more resistant to the treatment of silica nanoparticles than were macrophages [113]. 

Inbar and coworkers689 determined that [3H]-acetylated lectin did not bind to the surface of normal cell-lines (hamster and rat embryo, and mouse 3T3 cells) or to the transformed counterparts of these cells. Trypsinization did not alter these results. The authors concluded688 that L-fucosyl residues were not exposed on the cell surface. In view of the specificity studies of Kabat and coworkers,200,686 this interpretation may be incorrect L-fucosyl residues may be rendered sterically inaccessible by glycosylation of neighboring sugar residues. 

Human oral tumor cell line Human oral normal cell line ... 

Transformed and tumorigenic cells are different from normal cell lines in that they are not usually anchorage dependent. They exhibit a spherical shape, increased life span and lateral diffusivity of membrane proteins, decreased cell receptors and membrane proteins, and a different cytoskeletal structure. The decrease in the concentration of the cell adhesion molecules in the cell membrane of these cells causes the anchorage independence. Transformed cell lines also do not assemble a normal ECM. It is important to note that some cell lines (e.g., lymphocytes) that are normally anchorage dependent can be induced and then adapted to become anchorage independent. This is of tremendous importance to recombinant protein production as discussed later, because the scale-up of suspension cultures is easier than that of anchorage-dependent cell lines. 

As described in the section 3.1.1, the diterpenoids from S. dulcis showed relatively strong cytotoxicity against HaLa cells. In order to evaluate SDB as antitumor agent, the cytotoxic effects of SDB were examined in six human cell lines, i.e., HeLa 229, HeLa S3 and HEp-2 cells derived from tumor tissues and FL, Chang liver and intestine 407 cells derived from normal tissues. As summarized in Table 9, SDB showed potent cytotoxicity for the cell lines tested. It is notable that higher toxicity was observed in tumor cell lines than in normal cell lines. Similar tendency on the cell growth was also found when the effects of 5-fluorouracil (5-FU) on the cell growth of these cultures were examined. 

Table 7-5 Cytotoxicities of designed enediyne 357 against a panel of 21 tumor cell lines (top group) and four normal cell lines (bottom group)...

In summary, the tilorone polymers inhibit both RNA and DNA viruses and deserve further consideration as antivirial agent in the war against viruses and possible bioterrorism involving viruses. Further, they show good inhibition of virus replication in both transformed cell lines but also in normal cell lines, a condition that better mimics antiviral therapy of humans. 

Mackay AR, Ballin M, Pelina MD, Farina AR, Nason AM, Hartzler JL, et al (1992) Effect of phorbol ester and cytokines on matrix metalloproteinase and tissue inhibitor of metalloproteinase expression in tumor and normal cell lines. Invasion Metastasis 12 168-184... 

Borkird and Sung [1] isolated seven ABA-insensitive cell lines in a somatic embryo culture of carrot in which ABA failed to arrest the development of torpedo-stage embryos into plantlets, as occurs in wild type when ABA is added. All lines also showed reduced auxin sensitivity. Characterization of three lines showed lower levels of ABA uptake as a possible cause of ABA insensitivity. However, the uptake of 2,4-D was higher than in wild-type embryos. In tobacco, cell-lines resistant to inhibition of growth by ABA were also isolated [25], and cell proliferation continued in the presence of what would be growth-inhibiting ABA concentrations for normal cell lines. 

The tests were conducted on breast cancer cell lines (MDA-MB-231 and 468) and normal cell lines to study the selectivity. A total selectivity was observed toward infected cells. Complex 22 once again showed the most interesting cytotoxicity compared respectively to 28 and 29. The accumulation of gold in... 

There are a number of technical problems associated with biochemical studies on RARs. These include the fact that these proteins are not abundant in most cells and tissues, that high-specific activity retinoids will be required to detect binding and that high concentrations of salt are required for the solubilization of these proteins from nuclei and this may interfere with certain types of experiment. Higher levels of RAR expression may be obtained by transfection of cells with RAR cDNAs. RARs have been successfully extracted from transfected cells, although the subcellular localization may be different to that generally observed in normal cell lines (7). 

Sansalvamide A is an antineoplastic macrocyclic depsipeptide isolated from the marine fungus Fusarium by William Fenical in 1999. Studies have shown potent cytotoxicity against NCI s 60 cell line panel for the natural product and ten-fold inhibition over the depsipeptide for the pentapeptide derivative. A library of over 100 derivatives of the compound have been synthesized and the Structural Activity Relationships (SARs) show promising bio-potency against pancreatic, colon, breast, prostate and melanoma cancer cell-lines. Six derivatives show over 100-fold differential selectivity for cancer cell lines over normal cell lines and are over 100 times more active against pancreatic cancer cell lines than compounds used clinically to treat these cancers (e.g., 5-FU). Through use of inhibition assays and analysis of trends emerge that can be used to modify the scaffold to produce more potent compounds. 

In intact lymphocytes in culture, adenine inhibited the early steps of purine biosynthesis to the same degree in the mutant cell line as it did in the normal cell line. Hypoxanthine inhibited FGAR synthesis of the HGRPT deficient Lesch-Nyhan lymphocytes only in concentrations 30-40 times higher than in normal lymphocytes. 

These nanoparticles play a major role in ligand-free cellular uptake of oil-loaded nanoparticles. They reveal the ability to degrade polymers for nano-vectors in a site-specific manner. The qdotoxicity result helps us in the selection of drugs to the target sites of carcinoma cells. Here we can monitor the toxicity levels of normal cell line and cancer cell line, which helps us to monitor the toxicity levels both in vitro and in vivo. 

Marin, G., and Littlefield, J. W., 1968, Selection of morphologically normal cell lines from polyoma-transformed BHK 21/13 hamster fibroblasts, 7. Virology 2 69. 

---