Human peripheral blood mononuclear

Deguchi, Y., Negoro, S., Kishimoto, S. (1988). Age-related changes of heat shock protein gene transcription in human peripheral blood mononuclear cells. Biochem. Biophys. Res. Comm. 157, 580-584. 

Wetzel MA, Steele AD, Eisenstein TK, Adler MW, Henderson EE, Rogers TJ (2000) Mu-opioid induction of monocyte chemoattractant protein-1, RANTES, and IFN-gamma-inducible protein-10 expression in human peripheral blood mononuclear cells. J Immunol 165 6519-6524 Widmer U, Manogue KR, Cerami A, Sherry B (1993) Genomic cloning and promoter analysis of macrophage inflammatory protein (MIP)-2, MIP-1 alpha, and MIP-1 beta, members of the chemokine superfamily of proinflammatory cytokines. J Immunol 150 4996-5012 Ye RD (2001) Regulation of nuclear factor kappaB activation by G-protein-coupled receptors. [Review] [136 refs]. J Leukoc Biol 70 839-848... 

Chao CC, Molitor TW, Close K, Hu S, Peterson PK (1993) Morphine inhibits the release of tumor necrosis factor in human peripheral blood mononuclear cell cultures. Int J Immunopharmacol 15 447 53... 

Peterson PK, Sharp BM, Gekker G, Jackson B, Balfour HH Jr (1991) Opiates, human peripheral blood mononuclear cells, and HIV. Adv Exp Med Biol 288 171-178 Peterson PK, Gekker G, Schut R, Hu S, Balfour HH Jr, Chao CC (1993) Enhancement of HlV-1 replication by opiates and cocaine the cytokine connection. Adv Exp Med Biol 335 181-188 Peterson PK, Gekker G, Hu S, Sheng WS, Molitor TW, Chao CC (1995) Morphine stimulates phagocytosis of Mycobacterium tuberculosis by human microglial cells involvement of a G protein-coupled opiate receptor. Adv Neuroimmunol 5 299-309 Peterson PK, Molitor TW, Chao CC (1998) The opioid-cytokine connection. J Neuroimmunol 83 63-69... 

Studies have demonstrated that one such method is to examine the effects of disinfectants on endogenous RNA-dependent DNA polymerase (i.e. reverse transcriptase) activity. In essence, HIV is an RNA virus after it enters a cell the RNA is converted to DNA under the influence of reverse transcriptase. The virus induces a cytopathic effect on T lymphocytes, and in the assay reverse transcriptase activity is determined after exposure to different concentrations of various disinfectants. However, it has been suggested that monitoring residual viral reverse transcriptase activity is not a satisfactory alternative to tests whereby infectious HIV can be detected in systems employing fresh human peripheral blood mononuclear cells. 

Jyonouchi, H., Sun, S., and Gross, M., Effect of carotenoids on in vitro immunoglobulin production by human peripheral blood mononuclear cells astaxanthin, a carotenoid without vitamin A activity, enhances in vitro immunoglobulin production in response to a T-dependent stimulant and antigen, Nutr. Cancer, 23, 171, 1994. 

Kishimoto T, Oguri T, Ueda D, Tada M. 1996. Methylmercury modulation of monocyte chemotactic protein-1 mRNA expression in human peripheral blood mononuclear cells. Hum Cell 9 371-374. 

Lu Z, Zhang R, Diasio RB. Dihydropyrimidine dehydrogenase activity in human peripheral blood mononuclear cells and liver population characteristics, newly identified deficient patients, and clinical implication in 5-fluorouracil chemotherapy. Cancer Res 1993 53 5433-5438. 

Mounho, B.J., Davila, D.R., and Burchiel, S.W., Characterization of intracellular calcium responses produced by polycyclic aromatic hydrocarbons in surface marker-defined human peripheral blood mononuclear cells, Toxicol. Appl. Pharmacol., 145, 323, 1997. 

In vitro TGN1412 caused a profound, polyclonal T-cell proliferation of human peripheral blood mononuclear cells, including those from patients with BCLL. It also induced a profound activation and proliferation of T-cell subsets including CD4+ and CD8+ T cells, naive and memory T cells, and regulatory T cells. TGN1412 was shown to induce a transient, well tolerated expansion of T cells in nonhuman primates treated with TGN1412 and efficacy was demonstrated in a rhesus monkey collagen-induced arthritis model. 

Pisa, E.K. et al., OKT3-induced cytokine mRNA expression in human peripheral blood mononuclear cells measured by polymerase chain reaction, ScandL J. Immunol., 36, 745, 1992. 

Peterson, P.K. et al., Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures, AIDS, 4, 869, 1990. 

Rininger, J.A. et al., Immunopharmacological activity of Echinacea preparations following simulated digestion on murine macrophages and human peripheral blood mononuclear cells, J Leukoc Biol, 68, 503, 2000. 

Guo, T.L., Mudzinski, S.P. and Lawrence, D.A., The heavy metal lead modulates the expression of both TNF-a and TNF-a receptors in lipopolysaccharide-activated human peripheral blood mononuclear cells, J. Leuk. Biol. 59, 932, 1996. 

Sabharwal, P. et al., Prolactin synthesized and secreted by human peripheral blood mononuclear cells An autocrine growth factor for lymphoproliferation, Proc. Nat. Acad. Sci., 89, 7713, 1992. 

Varma, S. et al., Growth hormone secretion by human peripheral blood mononuclear cells detected by an enzyme-linked immunoplaque assay, J. Clin. Endocrinol. Metab., 76, 49, 1993. 

Chao, C.C. et al., Morphine potentiates transforming growth factor-beta release from human peripheral blood mononuclear cell cultures, J. Pharmaco.l Exp. Ther., 262, 19, 1992. 

Recently, it has been found that NO donors inhibit HIV-1 replication in acutely infected human peripheral blood mononuclear cells (PBMCs), and have an additive inhibitory effect on HIV-1 replication in combination with 3 -azido-3 -deoxythymisylate (AZT) [139, 140]. S-nitrosothiols (RSNOs) inhibit HIV-1 replication at a step in the viral replicative cycle after reverse transcription, but before or during viral protein expression through a cGMP-independent mechanism. In the latently infected U1 cell line, NO donors and intracellular NO production stimulate HIV-1 reactivation. These studies suggest that NO both inhibits HIV-1 replication in acutely infected cells and stimulates HIV-1 reactivation in chronically infected cells. Thus, NO donors may be useful in the treatment of HIV-1 disease by inhibiting acute infection, or reactivating a latent virus. 

Monti D, Moretti L, Salvioli S, Straface E, Malorni W, Pellicciari R, Schettini G, Bisaglia M, Pincelli C, Fumelli C, Bonafe M, Franceschi C (2000) C60 carboxyfullerene exerts a protective activity against oxidative stress-induced apoptosis in human peripheral blood mononuclear cells. Biochemical and Biophysical Research Communications 277 711-717. 

These reactions were demonstrated with pure enzymes or various biological media such as human peripheral blood mononuclear cells uninfected or infected with HIV. In the latter case, highly promising antiviral results were obtained. Furthermore, a variety of amino acyl residues, carboxylic acid ester moieties, and aryl substituents X have been reported. 

Schmitz, F., Schrader, H., Otte, J. M., et al. (2001) Identification of CCK-B/gastrin receptor splice variants in human peripheral blood mononuclear cells. Regul. Pept. 101, 25-33. 

Table 17.1 Immune response genes expressed at moderate (+) or high (++) levels at 12h in human peripheral blood mononuclear cells after in vitro restimulation with pertussis toxin antigen for 4,12, 24, and 48 h...

T-cells influence. SSTE and nicotine were incubated in splenic mononuclear cells at concentrations of 1 10 or 1 10 dilutions of STD, or 10 or 100 i.g/mL nicotine, during 4 days of stimulation with anti-CD3. The treatment sustained expression of IL-2, IFN-y, IL-10, and IL-4 cytokine mRNA at 100 i.g/mL nicotine. STD did not exhibit residual expression of cytokine mRNA. Restimulated STD exhibited maximum IL-2, IL-4, IFN- y, and IL-10 mRNA at 48 hours . STE, in splenic mononuclear cell culture at LlOHo 1 10 dilutions, increased IL-2 production and decreased IL-10 at 1 10 dilution. IFN-y production was decreased at all concentrations. STE did not alter IL-4 product ion k P-benzoquinone, a thiol-reactive benzene derivative from cigarette tar, was incubated in human peripheral blood mononuclear cells at a concentration of 10 xM. The treat-... 

Coumarin was not toxic at concentrations up to 100 pg/mL to human peripheral blood mononuclear cells in vitro. Coumarin and its metabolite, 7-hydroxycoumarin, produced significant suppression of human bone marrow progenitor stem cell activity at concentrations greater than 200 pg/mL, whereas coumarin concentrations of 25 pg/mL and above produced suppression of murine bone marrow progenitor stem cell activity (Gallicchio et al., 1989). 

Gallicchio, V.S., Hulette, B.C., Harmon, C. Marshall, M.E. (1989) Toxicity of coumarin (1,2-benzopyrone) on human peripheral blood mononuclear cells and human and murine bone marrow progenitor stem cells. J. biol. Res. Modifiers, 8, 116-121 Gangolh, S.D., Shilling, W.H., Grasso, P. Gaunt, I F. (1974) Studies on the metabolism and hepatotoxicity of coumarin in the baboon. Biochem. Soc. Transact., 2, 310-312... 

J.B. Matthews, T.R. Green, M.H. Stone, B.M. Wroblewski, J. Fisher, and E. Ingham, Comparison of the response of primary human peripheral blood mononuclear phagocytes from different donors to challenge with model polyethylene particles of known size and dose, Biomaterials, 21(20) 2033-2044, 2000. 

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