Genomic cloning

Gopalapillai, R., Kadono-Okuda, K., Tsuchida, K. et al. 2006. Lipophorin receptor of Bombyx mori cDNA cloning, genomic structure, alternative splicing, and isolation of a new isoform. J. Lipid Res., 47(5) 1005-1013. 

Helps find promocers by cloning genomic DNA and seleccing for Cmr... 

Indik, Z., Yeh, H., Ornstein-Goldstein, N., Sheppard, P., Anderson, N., Rosenbloom, J. C., Peltonen, L., and Rosenbloom, J. (1987). Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA. Proc. Natl. Acad. Sci. USA 84, 5680-5684. 

Southard-Smith M, Pierce JC, MacDonald RJ. Physical mapping of the rat tissue kallikrein family in two gene clusters by analysis of PI bacteriophage clones. Genomics 1994 22 404-417. 

I. -S., Lesnick, J.D., Ford, A.P.D.W., Daniels, D.V., Eglen, R.M., Clarke, D.E., Bach, C., Chan, FEW. Molecular cloning, genomic characterization and expression of novel human aiA-adrenoceptor isoforms. FEBS Lett. 1998, 422, 279-283 ... 

De Lucchini S, Marracci S, Nardi I. The serotonin 5-HT2B receptor from the puffer fish Tetraodon fluviatilis cDNA cloning, genomic organization and alternatively spliced variants. Brain Res Mol Brain Res 2001 97 89-93. 

Landegent J E, Jansen in de Wal N, Dirks R W, Baas F and van der Ploeg M. Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Hum Genet 77 366-70, 1987. 

Henneberry, A.L., and McMaster, C.R., 1999, Cloning and expression of a human choline/ethanol-aminephosphotransferase synthesis of phosphatidylcholine and phosphatidylethanolamine. BiochemJ. 339 291-298 Henneberry, A.L., Wistow, G., and McMaster, C.R., 2000, Cloning, genomic organization, and characterization of a human cholinephosphotransferase. J. Biol. Chem. 275 29808-29815... 

De Miranda J, Santoro A, Engelender S, and Wolosker H (2000) Human serine racemase molecular cloning, genomic organization and functional analysis. Gene 256,183-8. 

Humphries DE, Lanciotti J, Karlinsky JB. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl A/-deacetylase/A/-sulphotransferase-2. Biochem. J. 1998 332(2) 303-307. 

PI clones Genomic sequences 90 kb Genes Mapping Interphase cytogenetics Usually make good FISH probes High... 

Marquardt, A., Stohr, H., White, K., and Weber, B. H. cDNA cloning, genomic structure, and chromosomal locaUzation of three members of the human fatty acid desaturase family. Genomics 66 (2000) 175-183. 

The presence of ACC synthase isozymes was proved by cloning of cDNAs with different nucleotide sequences from tomato and winter squash mRNA libraries, and also by expression studies with cloned genomic fragments from Arabidopsis thaliana and rice (see Section 5.2.5). 

Gebe, J.A., Llewellyn, M., Hoggatt, H., and Amffo, A., 2000, Molecular cloning, genomic organization and cell-binding characteristics of mouse Spalpha. Immunology 99 78-86. 

FIGURE 3. (right) Transcription of the cloned genomic region as analyzed by Northern hybridization. RNA (10 pg) was electrophoresed on a 1% agarose gel containing 0.7 M formaldehyde, tr s-ferred to Zeta-Probe (BioRad) and hybridized with the P-labeled 0.87 kb PstI fragment. The RNA was prepared from cells exposed to I0W-CO2 for 2 hours. 

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