Cytokine mRNA expression

Varney VA. Hamid QA. Gaga M. Ying S. Jacobson M, Frew AJ. Kay AB. Durham SR Influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses. J Clin Invest 1993 92 644-651. 

Pisa, E.K. et al., OKT3-induced cytokine mRNA expression in human peripheral blood mononuclear cells measured by polymerase chain reaction, ScandL J. Immunol., 36, 745, 1992. 

Zhou, H. R., Yan D., and Pestka J.J. Differential cytokine mRNA expression in mice after oral exposure to the trichothecene vomitoxin (deoxynivalenol) Dose response and time course. Toxicol. Appl. Pharmacol. 144, 294, 1997. 

Cytokine profiling has also been measured as a function of changes in cytokine mRNA expression using either reverse transcription polymerase chain reaction (RT-PCR) [87, 91-93] or ribonuclease protection assay (RPA) [94-97], Measurement of cytokine transcripts by RT-PCR revealed that prolonged exposure to TMA induced increased levels of IL-4 mRNA expression compared with treatment with DNCB [87,92-93]. However, expression of the type 1 cytokine IFN-y by DNCB-activated LNC was variable and failed to discriminate between contact and respiratory allergens [87,91,93). A similar profile was observed for freshly isolated tissue analyzed by RPA. This somewhat less... 

Wynn, T.A., Eltoum, I., Cheever, A.W., Lewis, F.A., Cause, W.C. and Sher, A. (1993) Analysis of cytokine mRNA expression during primary granuloma formation induced by eggs of Schistosoma mansoni. The Journal of Immunology 151,1 430-1 440. 

Rabinovitch, A., Suarez-Pinzon, W. L., Sorensen, O., Bleackley, R. C., Power, R. F. and Rajotte, R. V. (1995). Combined therapy with interleukin-4 and interleukin-10 inhibits autoimmune diabetes recurrence in syngeneic islet-transplanted nonobese diabetic mice. Analysis of cytokine mRNA expression in the graft.Transplantation 60, 368-374. 

Chen, T, Shen, E, Zhang, I, and Hua, Z. 2005. Effects of microcystin-LR on patterns of iNOS and cytokine mRNA expression in macrophages in vitro. Wiley Periodicals Inc Env Toxicol 20 85-91. 

Zhou, H.R., Islam, Z., Pestka, J.J. (2003a). Rapid, sequential activation of mitogen-activated protein kinases and transcription factors precedes proinflammatory cytokine mRNA expression in spleens of mice exposed to the trichothecene vomitoxin. 

Kirkham B, Portek I, Lee C S et al 1999 Intraarticular variability of synovial membrane histology, immunohistology and cytokine mRNA expression in patients with rheumatoid arthritis. Journal of Rheumatology 26 777-784... 

A. B. and Durham, S.R (1993). Increases in activated T lymphocytes, eosinophils and cytokine mRNA expression for interleukin-5 and granulocyte/macrophage colony-stimulating factor in bronchial biopsies after allergen inhalation challenge in atopic asthmatics. Am. J. Respir. Cell Mol. Biol. 8, 35-42. 

Okayama, Y. and Church, M.K. (1995). Multiple cytokine mRNA expression in human mast cells stimulated via FCfRI. Int. Arch. Allergy Immunol, in press. 

Graham BS, Henderson GS, Tang YW, Lu X, Neuzil KM, Colley DG. Priming immunization determines T helper cytokine mRNA expression patterns in lungs of mice challenged with respiratory syncytial virus. J Immunol. 1993 151(4) 2032-2040. 

Kennedy, M. K., Torrance, D. S., Picha, K. S., and Mohler, K. M., Analysis of cytokine mRNA expression in the central nervous system of mice with experimental autoimmune encephalomyelitis reveals that IL-10 mRNA expression correlates with recovery, J. Immunol., 149, 2496, 1992. 

Woodroofe, M. N. and Cuzner, M. L., Cytokine mRNA expression in inflammatory multiple sclerosis lesions detection by non-radioactive in situ hybridization, Cytokines, 5, 583, 1993. 

Puffenbarger RA, Boothe AC, Cabral GA (2000) Cannabinoids inhibit LPS-inducible cytokine mRNA expression in rat microglial cells. Glia 29 58-69... 

ROBINSON, D.S., HAMID, Q BENTLEY, A., YING, S KAY, A.B. DURHAM, S.R. (1993) Activation of CD4+ T cells, increased Th2-type cytokine mRNA expression and eosinophil recruitment in bronchoalveolar lavage after allergen inhalation challenge in patients with atopic asthma. Journal of Allergy and Clinical Immunology, 92, 313-324. 

Gaspard I, Kerdine S, Paiiardy M, Lebrec H. Quantitation of cytokine mRNA expression as an endpoint for prediction and diagnosis of xenobiotic-induced hypersensitivity reactions. Methods 1999 19 64-70. 

Figure 9 RT-PCR analysis of cytokine mRNA expression in the spleens of DBA/2 mice immunized with casein/CKA. Total RNA was extracted from pooled spleens of S DHA/2 mice or CVE-administercd mice 3 weeks after immunized using RNAzol and reverse-transcribed, and the cDNA concentration was adjusted by co-amplification of two fold serially diluted cDNA and constant amounts of control plasmid pMCQ with [5-actin specific primers. Cytokine expression patterns of IL-12, IFNy, IL-6, and 11.-10 were determined by PCR using murine cytokine-specific primers. The PCR products were separated in 2 % agarose gel and visualized by ethidium bromide staining. Relative quantification of the amounts of the RT-PCR products was performed using a Computing Densitometer and NIH Image software,...

Harris, P, Smythies, L., Smith, P. and Dubois, A. 2000. Inflammatory cytokine mRNA expression during early and persistent Helicobacter pylori infection in nonhuman primates. J. Infect. Dis. 181 783-786. 

Shi MM, Godleski JJ, Paulauskis ID. Effect of oxidative stress on inflammatory cytokine mRNA expression in rat alveolar macrophages. FASEB J 1995 9 A346. 

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