Human carcinoembryonic antigen

Human carcinoembryonic antigen Mouse/human chimeric IgGl antibody (cT84.66) Antibody - mediated cancer therapy (colon cancer, breast cancer and tumor with epithelial origin) N. tabacum cv petit Havana SRI (Transient expression) 35S MSP + 1 mg/kg FLW 42... 

CEA-scan (Arcitumomab, murine monoclonal antibody (Mab) fragment (Fab), directed against human carcinoembryonic antigen, CEA)... 

K. H. Lan, F. Kanai, Y. Shiratori, M. Ohashi, T. Tanaka, T. Okudaira, Y. Yoshida, H. Hamada, and M. Omata, In vivo selective gene expression and therapy mediated by adenoviral vectors for human carcinoembryonic antigen-producing gastric carcinoma, Cancer Res. 57 4279 (1997). 

Rowe HA, Griffiths NJ, Hill DJ, Virji M (2007) Co-ordinate action of bacterial adhesins and human carcinoembryonic antigen receptors in enhanced cellular invasion by capsulate serum resistant Neisseria meningitidis. Cell Microbiol 9 154-168... 

Horig H, Lee DS, Conkright W, Divito J, Hasson H, LaMare M, Rivera A, Park D,Tine J, Guito K,Tsang KW, Schlom J, Kaufman HL. Phase I clinical trial of a recombinant canarypoxvirus (ALVAC) vaccine expressing human carcinoembryonic antigen and the B7.1 co-stimulatory molecule. Cancer Immunol Immunother 2000 49 504-514. 

An example of the application of the microchip-based immunosorbent assay is shown below. The human carcinoembryonic antigen (CEA), one of the most widely used tumor markers for serodi-agnosis of colon cancer, was assayed with a microchip-based system [9]. An ultratrace amount of CEA dissolved in serum samples was successfully determined within a short time. Polystyrene beads pre-coated with anti-CEA antibody were introduced into a microchannel. Then a serum sample containing CEA, the first antibody, and the second antibody conjugated with colloidal gold. 

Drewinko B, Romsdahl MM, Yang LY, Ahearn MJ, Tmjillo JM. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 1976 36 467-75. 

J3. Jessup, J. M., and Thomas, P. C., Carcinoembryonic antigen Function in metastasis by human colorectal carcinoma. Cancer Metastasis Rev. 8, 263-280 (1989). 

Ohannesian, D.W., et al. 1994. Concomitant increases in galectin-1 and its glycoconjugate ligands (carcinoembryonic antigen, lamp-1, and lamp-2) in cultured human colon carcinoma cells by sodium butyrate. Cancer Res 54 5992. 

Carcinoembryonic antigen (CEA) 2001 A Circulating CEA in human sera 92.3% of patients with high CEA levels and 50% of patients with normal CEA levels were found positive Ren et al. [18], use of a bispecific CEAScFv-streptavidin fusion protein-based immuno-PCR technique (see Section 2.1.1)... 

T. Osaki, Y. Tanio, I. Tachibana, S. Hosoe, T. Kumagai, I. Kawase, S. Oikawa, and T. Kishimoto, Gene therapy for carcinoembryonic antigen-producing human lung cancer cells by cell type-specific expression of herpes simplex virus thymidine kinase gene, Cancer Res. 54 5258 (1994). 

In another Eu-Tb two-color immunoassay for two low concentration components, highly luminescent labels, BPTA-Tb3+ and BHHCT-Eu3+ were used for human serum AFP and CEA (carcinoembryonic antigen) by Yuan et al. (2001) (fig. 12). As shown in fig. 10, a well was coated with AFP and CEA antibodies, and after the serum sample was applied, anti-AFP antibody labeled with BHHCT-Eu3+ and anti-CEA antibody labeled with biotin were reacted. After washing the well and measuring the emission of the Eu chelate at 615 nm, SA labeled with BPTA-Tb3+ was added and the emission of the Tb chelate at 545 nm was measured. In this system, the detection limits of AFP and CEA were very low, 44 pg/ml and 76 pg/ml, respectively. 

The oncolytic viruses include adenovirus, measles, reovirus, vesicular stomatitis virus (VSV),HSV,poxvirus, and vaccinia. Specific examples include (1) ONYX-015, which is an adenoviral oncolytic virus, administered to patients with liver metastases of colorectal cancer and pancreatic cancer [29], (2) Reolysin, which is an oncolytic reovirus administered to patients with glioma [30], and (3) MV-CEA, which is an oncolytic measles virus expressing carcinoembryonic antigen, administered to patients with ovarian cancer [31]. Some oncolytic viruses are wild type and are apparently not pathogenic in humans, such as the Newcastle disease virus (NDV), which is an RNA avian paramyxovirus. PV701, a naturally attenuated, replication-competent strain of NDV, has been administered to patients with advanced solid tumors [32], The applicability of oncolytic viruses as a therapy for clinical oncology trials is due to their potential selectivity the ability to kill tumor cells but not normal cells. However, the level of attenuation of viral replication in normal cells is limited for most oncolytic vectors. 

Sato K, Tokeshi M, Kimura H, Kitamori T. Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoassay in a microchip for cancer diagnosis. Anal Chem 2001 73 1213-1218. 

Carcinoembryonic Antigen (CEA). Twelve disposable glass test tubes (1.5 X 15 cm, Packard Instrument Co.) are labeled appropriately, and 5 ml of EDTA buffer (3.7 mM EDTA, pH 6.5, 0.017% sodium azide, and 0.002% BSA) are added to each tube. Two-hundred microliters of 50 mM borate buffer, pH 8.4, containing 10% group A human plasma and 0.01% thimerosal are added to the two blank tubes, and 2(K) ju.1 of five increasing dilutions (appropriate dilutions must be determined experimentally for each antiserum) in the same buffer of anti-CEA serum are added in dupli-... 

Buchegger, F. Haskell, C.M. Schreyer, M. Scazziga, B.R. Randin, S. Carrel, S. Mach, J.-P. Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice. J. Exp. Med. 1983, 158, 413 27. 

CEA, Carcinoembryonic antigen AFP, alpha fetoprotein hCG, human chorionic gonadotropin. 

Modified from Sell S. Cancer markers. In Moossa AR, Schempff SC, Robson MC, eds. Compreliensive textbook of oncology, 2nd ed, Vol. 1, Baltimore Williams Wilkins, 1991 225-38. hCG, Human chorionic gonadotropin ACTH, adrenocorticotropic hormone AFP, alpha fetoprotein CEA, carcinoembryonic antigen. 

Sutherland, R. M., Buchegger, F Schreyer, M Vacca, A., and Mach, J. P. (1987) Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular spheroids. Cancer Res. 47, 1627-1633. 

Sharkey, R. M., Primus, F. J., Shochat, D., and Goldenberg, D. M. (1988) Comparison of tumour targeting of mouse monoclonal and goat polyclonal antibodies to carcinoembryonic antigen in the GW-39 human tumour-hamster host model. Cancer Res. 48, 1823-1828... 

Rosenthal, K. C., Tompkins, W. A. F., and Rawls, W. E. (1980) Factors affecting the expression of carcinoembryonic antigen at the surface of cultured human colon carcinoma cells. Cancer Res. 40,4774-4750. 

Shi, Z. R., Tsao, D., and Kim, Y. S. (1983) Subcellular distribution, synthesis, and release of carcinoembryonic antigen in cultured human colon adenoma cell lines. Cancer Res. 43,4045-4049. 

Ravindranath MH, Shen P, Habal N, et al. Does human melanoma express carcinoembryonic antigen Anticancer Res. 2000 20 3083-3092. 

Svenberg T. Carcinoembryonic antigen-like substances of human bile. Isolation and partial characterization. Int J Cancer. 1976 17 588-596. 

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