Luminescent label

The possibility of conformational changes in chains between chemical junctions for weakly crosslinked CP in ionization is confirmed also by the investigation of the kinetic mobility of elements of the reticular structure by polarized luminescence [32, 33]. Polarized luminescence is used for the study of relaxation properties of structural elements with covalently bonded luminescent labels [44,45]. For a microdisperse form of a macroreticular MA-EDMA (2.5 mol% EDMA) copolymer (Fig. 9 a, curves 1 and 2), as compared to linear PM A, the inner structure of chain parts is more stable and the conformational transition is more distinct. A similar kind of dependence is also observed for a weakly crosslinked AA-EDMA (2.5 mol%) copolymer (Fig. 9b, curves 4 and 5). 

Other immunoassays are based on the same antibody-antigen binding reaction but use a different labeling system for detection. Instead of an enzyme label, there are radioactive isotopes, and fluorescent and luminescent labels. Some important immunoassays are defined below ... 

Label selection will also affect assay sensitivity and, sometimes, selectivity. Radioactive tracers, luminescent labels, or enzymes have been applied for MIP-ILAs development and will be described in more detail in the next sections. 

Imaging of DNA and Protein Arrays with the Help of Luminescent Labels... 

Takuya Nishioka, Koichi Fukui and Kazuko Matsumoto, Lanthanide chelates as luminescent labels in biomedical analyses 171... 

LANTHANIDE CHELATES AS LUMINESCENT LABELS IN BIOMEDICAL ANALYSES... 

It naturally occurred to mind that if a luminescent label is developed, a much simpler and faster analysis would be at hand. Actually, such luminescent Eu3+ labeling was developed by Diamandis (1988), Diamandis and Christopoulos (1990). The structure of the fluorescent label (Eu3+-4,7-bis(chlorosulfophcnyl)-l,10-phenanthrolinc-2,9-dicarboxylatc, BCPDA-Eu3+) is... 

Photophysical properties of the lanthanide luminescent labels described in scheme 13 and bound to protein... 

In another Eu-Tb two-color immunoassay for two low concentration components, highly luminescent labels, BPTA-Tb3+ and BHHCT-Eu3+ were used for human serum AFP and CEA (carcinoembryonic antigen) by Yuan et al. (2001) (fig. 12). As shown in fig. 10, a well was coated with AFP and CEA antibodies, and after the serum sample was applied, anti-AFP antibody labeled with BHHCT-Eu3+ and anti-CEA antibody labeled with biotin were reacted. After washing the well and measuring the emission of the Eu chelate at 615 nm, SA labeled with BPTA-Tb3+ was added and the emission of the Tb chelate at 545 nm was measured. In this system, the detection limits of AFP and CEA were very low, 44 pg/ml and 76 pg/ml, respectively. 

---