Microchip-based

Huynh, B.H., Fogarty, B. A., Martin, R.S., Lunte, S.M. (2004). On-line coupling of microdialysis sampling with microchip-based capillary electrophoresis. Anal. Chem. 76, 6440-6447. 

Sato K, Mawatari K, Kitamori T (2008) Microchip-based cell analysis and clinical diagnosis system. Lab Chip 8 1992-1998... 

J. Wang, A. Ibanez, and M.P. Chatrathi, Microchip-based amperometric immunoassays using redox tracers. Electrophoresis 23, 3744—3749 (2002). 

Horseradish peroxidase HRP-catalyzed Luminol- H202 Borate buffer (pH 8.5) Microchip-based CE coupled with CL detection 7-35 nM (for HRP) 107... 

Solid-supported technologies are already well established methods in medicinal chemistry and automated synthesis. Over the last couple of years new trends have evolved in this field which are of utmost importance as they have the potential to revolutionize the way chemical synthesis especially for library production is performed. Microchip-based synthesis technologies and multistep sequences with solid-supported catalysts or reagents in flow-through systems are only two spectacular examples. A new approach is the use of solid-supported systems for the scale-up of chemical reactions thereby enabling the rapid and smooth transition from discovery to development units. 

Sundberg, S.A., Chow, A., Nikiforov, T., and Wada, H.G., Microchip-based systems for target validation and HTS, Drug Discov. Today, 5, 92, 2000. 

XVIII. COUPLING CE AND MICROCHIP-BASED DEVICES WITH MASS SPECTROMETRY... 

The hyphenation of CE with techniques allowing structural determination of impurities is the object of Chapter 18 Coupling CE and Microchip-based Devices and Mass Spectrometry of this book, but the potential of CE coupled to mass spectrometry (CE/MS) in impurity profiling has also been discussed elsewhere. ... 

Liu, C., Cui, D., Cai, H., Chen, X., and Geng, Z. (2006). A rigid poly(dimethylsiloxane) sandwich electrophoresis microchip based on thin-casting method. Electrophoresis 27, 2917—2923. 

J. C. Ackerman, G. S. Sheldon A microchip-based multi-nozzle nanoelectrospray device, in Proceedings of the 50th ASMS Conference on Mass Spectrometry and Allied Topics,... 

Mitchell MC, Spikmans V, de Mello AJ (2001) Microchip-based synthesis and analysis control of multicomponent reaction products and intermediates. Analyst 126(1 ) 24—27... 

Figure 13.9 Microchip-based micellar electrokinetic chromatography (MEKC) electro-pherogram of a mixture of nitroaromatics and nitramines. Analytes 20 ppm of each (1) TNB, (2) DNB, (3) NB, (4) TNT, (5) tetryl, (6) 2,4-DNT, (7) 2,6-DNT, (8) 2-, 3-, and 4-NT, (9) 2-Am-4,6-DNT, (10) 4-Am-2,6-DNT. Conditions MEKC buffer, 50 mM borate, pH 8.5, 50 mM SDS, 5 M Cy7, separation voltage 4 kV, separation distance 65 mm. (Reprinted in part with permission from [37]. Copyright 2000 American Chemical Society.)...

NH Chiem, DJ Harrison. Monoclonal antibody binding affinity determined by microchip-based capillary electrophoresis. Electrophoresis 19 3040-3044, 1998. 

Several successful attempts were done to transfer classical CEIA to a microchip-based format. This kind of miniaturization is a trend that can overcome the limitations of CE in high-throughput systems. On-chip CE offers both parallel analysis of samples and short separation times. Koutny et al. showed the use of an immunoassay on-chip (32). In this competitive approach fluorescein-labeled cortisol was used to detect unlabeled cortisol spiked to serum (Fig. 8). The system showed good reproducibility and robustness even in this problematic kind of sample matrix. Using serum cortisol standards calibration and quantification is possible in a working range of clinical interest. This example demonstrated that microchip electrophoretic systems are analytical devices suitable for immunological assays that can compete with common techniques. 

Monoclonal antibody-binding affinity was determined by microchip-based capillary electrophoresis with LIF detection (33). The mixing was carried out off-chip, and the on-chip separations were performed in less than 60 s (Fig. 9). A Scatchard plot analysis resulted in an affinity constant for the monoclonal anti-BSA antibody to fluorescently labeled BSA (BSA ) of... 

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