Multicellular spheroids

Runz-Schughart LA, Freyer JP, Hofetaedter F et al (2004) The use of 3-D cultures for high-throughput screening the multicellular spheroid model. J Biomol Screen 9 273-285... 

Matsushita, T., Ijima. H., Koide, N., Funatsu, K., High albumin production by multicellular spheroids of adull rat hepatocytes formed in the pores of polyurethane foam. Appl. Microbiol. Biotechnol. 1991. vol. 36. no. 3. pp. 324-326. 

Synthetic nanofiber matrices can provide physically and chemically stable 3D surfaces for ex vivo growth of cells. Meiners and coworkers showed that fibroblasts or rat kidney cells that have been grown on electrospun polyamide nanofiber meshes displayed all the characteristics of their counterparts in vivo [205], In addition, breast epithelial cells underwent morphogenesis to form multicellular spheroids containing lumens. 

In particular, malignant human ovary and mammary carcinomas can form solid aggregates with histological similarities to the primary tumours (Schleich, 1967). In addition, the production of extracellular matrix components similar to tumours in vivo has been demonstrated by Glimelius et al (1988) with multicellular spheroids of human glioma cells. 

Sutherland, R. M., Buchegger, F Schreyer, M Vacca, A., and Mach, J. P. (1987) Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular spheroids. Cancer Res. 47, 1627-1633. 

The multicellular spheroids were initiated by the agar overlay method described by Yuhas. After 6 days in culture, spheroids with diameters between 200 pm and 300 pm were selected for growth experiments and transferred to 16mm multiwell culture dishes. The diameters of the spheroids were determined by using an inverted microscope with a calibrated graticule in the eyepiece. The wells were base-coated with 0.5ml 0.75% DMEM-agar and filled with 1 ml of medium. 

It was also of interest to examine the effect of TTA on growth of human multicellular spheroids (D-54Mg). Figure 2 shows that the growth inhibitory effect of lOOpM TTA was evident after 7 days in culture. After 14 days in culture, TTA reduced the average spheroid diameter by approximately 38%, compart to control. 

V.H. Ho, N.K.H. Slater, R. Chen, Ph-responsive endosomolytic pseudo-peptides for drug delivery to multicellular spheroids tumour models, Biomaterials 32 (2011) 2953-2958. 

Single particles like cells or multicellular spheroids... 

Impedance Detection, Fig. 37 Placement of a multicellular spheroid in a pipette with a diameter 50 pm. T Teflon, 5 spheroid, M medium, P pipette... 

Lin RZ, Qiang HY. Recent advances in three-dimensional multicellular spheroid culture for biomedical research. Biotechnol J 2008 3 1172-1184. 

One type of 3D systems is based on the guided self-formation of multicellular spheroids. Such spheroids have been used primarily with liver and renal cells. One example of the use of such cultures is the study of gliadin toxicity [Elli et al, 2003]. In addition, Goodwin et al. [2000] have constructed a 3D model for assessment of in vitro toxicity in Balaena mysticetus renal tissue. 

Ho, V. H. B., Slater, N. K. H. and Chen, R. (2011). pH-responsive endosomolytic pseudo-f)eptides for drug dehvery to multicellular spheroids tumour models. Biomaterials, 32,2953-2958. 

Gdrlach A, Fandrey J, Holtermann G, Acker H. Effects of cobalt on haem proteins of erythropoietin-producing HepG2 cells in multicellular spheroid culture. FEBS Lett 1994 348(2) 216-218. 

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