Colon adenocarcinoma cells

A 2-h incubation with another PGE2 analogue, nocloprost (9P-chloro-DMPG) protects normal human fibroblasts but has no effect on the survival of colon adenocarcinoma cells exposed to 10 Gy (1000 rad) (218). Nocloprost protects against radiation-induced DSBs in normal cells but not in tumor cells. Moreover, incubation using nocloprost for 2 h after irradiation enhances the rate of DSB rejoining in fibroblasts but not in adenocarcinoma cells. These data possibly reflect a different distribution of PG receptors on the plasma membrane of the two cell types. 

PALOZZA P, SERINI S, MAGGIANO N, ANGELINI M, BONINSEGNA A, DI NICUOLO F, RANELLETTI F O and CALVIELLO G (2002) Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by P-carotene through down-regulation of cyclin A and Bcl-2 family proteins , Carcinogenesis, 23, 11-18. 

CCR2 expression was also examined in liver tumor formation induced by intraportal injection of a murine colon adenocarcinoma cell line (58). Tumor growth occurred at similar rates in wild-type and CCR2 /" mice, but the number... 

O Sullivan SM, Woods JA, and O Brien NM. 2004. Use of Tween 40 and Tween 80 to deliver a mixture of phytochemicals to human colonic adenocarcinoma cell (CaCo-2) monolayers. British Journal of Nutrition 91(5) 757-764. 

Palozza, P, S Serini, A Torsello et al. 2003. Beta-carotene regulates NF-kappa B DNA-binding activity by a redox mechanism in human leukemia and colon adenocarcinoma cells. J Nutr 133 381-388. 

In a recent study, the antiproliferative effect of different carotenoids, including (3-carotene, lycopene and lutein, on PCNA and cyclin Dl expression in human KB cells have been studied. The results indicate that carotenoids suppressed cell growth by acting as inhibitors of the expressions of PCNA and cyclin Dl, although in a different extent (Cheng et al., 2007). On the other hand, (3-carotene was able to induce a cell cycle delay in G2/M phase by decreasing the expression of cyclin A in human colon adenocarcinoma cells (Palozza et al., 2002a). 

Palozza, P., Serini, S., Boninsegna, A. et al. 2007a. The growth-inhibitory effects of tomatoes digested in vitro in colon adenocarcinoma cells occur through down regulation of cyclin DI, Bcl-2 and Bcl-xl. Br J Nutr 98 789-795. 

Yan, F., Subramanian, B., Nakeff, A., Barder, T.J., Parus, S.J., Lubman, D.M. (2003). A comparison of drug-treated and untreated HCT-116 human colon adenocarcinoma cells using a 2-D liquid separation mapping method based on chromatofocusing pi fractionation. Anal. Chem. 75, 2299-2308. 

Zhang, Z., Krylov, S., Arriaga, E.A., Polakowski, R., Dovichi, N.J. (2000). One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell. Anal. Chem. 72, 318-322. 

FIGURE 1.2 2D liquid protein expression map of the HCT-116 human colon adenocarcinoma cell line. (See text for full caption.)... 

Human colon adenocarcinoma cell line used as an absorption model... 

Y., Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2),... 

Peters, W. H. N., Roelofs, H. M. J., Time-dependent activity and expression of glutathione S-transferases in the human colon adenocarcinoma cell line Caco-1, Biochem. J. 1989, 264, 613-616. 

The human colon adenocarcinoma cell line (Caco-2) assay is still commonly used to measure the potential for a compound to be absorbed. It measures the permeability potential because permeability is a component of the absorption process,3 27 31 Multiple reports discuss the use of HPLC/MS/MS to support the Caco-2 assay.32 38... 

Caco-2 human Caucasian colon adenocarcinoma cells... 

Pinto, M., Robine-Leon, S., Appay, M.D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, and Zweibaum, A., Enterocytic-like differentiation and polarization of the human colon adenocarcinoma cell line Caco-2 in culture, Biol. Cell, 47,... 

Hirohashi, T., Suzuki, H., Chu, X.-Y., Tamai, I., Tsuji, A., and Sugiyama, Y., Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2), /. Pharmacol. Exp. Ther., 292, 265, 2000. 

Recently, Oryza sativa L. japonica cv. Dongjin cell cultures were used to produce an antibody against the tumor-associated glycoprotein 72 (TAG 72). TAG 72 is commonly expressed in human adenocarcinoma cells. Recombinant antibody purified from transgenic rice cells was shown to bind human LS 174T colon adenocarcinoma cells expressing TAG 72. This proved the ability of rice cells to produce a functional foreign protein. 

Fig. 2. Concentration-dependent inhibition of the growth of murine L1210 leukemia and rat RCC-2 colon adenocarcinoma cell populations by VBL ( ), deoxy VBL (O), epideoxy VBL ( ), and deoxydesethyl VBL (A).

OmuraM,TorigoeS,KubotaN. SN-38, ametabolite of the camptothecin derivative CPT-11, potentiates the cytotoxic effect of radiation in human colon adenocarcinoma cells grown as spheroids. Radiother Oncol 1997 43(2) 197-201. 

Holgate ST (1999) The epidemic of allergy and asthma. Nature 402(Suppl 6760) B2-B4 Hoskin DW, Reynolds T, Blay J (1994a) Colon adenocarcinoma cells inhibit anti-CD3-activated killer cell induction. Cancer Immunol Immunother CII 38(3) 201-207 Hoskin DW, Reynolds T, Blay J (1994b) Adenosine as a possible inhibitor of killer T-cell activation in the microenvironment of solid tumours. Int J Cancer 59(6) 854-855 Hoskin DW, Reynolds T, Blay J (1994c) 2-Chloroadenosine inhibits the MHC-unrestricted cytolytic activity of anti-CD3-activated killer cells evidence for the involvement of a non-Aj/ A2 cell-surface adenosine receptor. Cell Immunol 159(1) 85—93 Hoskin DW, Buttler JJ, Drapeau D, Haeryfar SM, Blay J (2002) Adenosine acts through an A3 receptor to prevent the induction of murine anti-CD3-activated killer T cells. Int J Cancer 99(3) 386—395... 

Caco-2 cells and isolated small intestine are models of basic nutrition that contributed to the understanding of resveratrol absorption and bioavailability. While the Caco-2 absorption model is a well-defined cellular in vitro system based on a human colonic adenocarcinoma cell line, the isolated small intestine model is nearer to in vivo conditions and is also simpler to handle. It also avoids the methodological problems of in vivo perfusion models [Barthe et al., 1998, 1999]. 

Figure 20.3 Heterogeneous distribution of liposomes in tumor tissues. Human colon adenocarcinoma cells (LS174T) were transplanted in dorsal skinfold chambers in severe combined immunodeficient mice. Fifteen to 32 days post tumor cell transplantation, fluorescently labeled liposomes were injected intravenously. The photos were taken at two days post injections. Liposomes accumulated only in perivascular regions in solid tumors. The arrows indicate liposomes internalized by cells. Iiar=100um. Reproduced with permission (Yuan etal., 1994).

Fujianmycins A and B were isolated from a Streptomyces species (IA-CAS-114) in Fujian, China [167], The structure of fujianmycin A (34a) may be regarded as the 2,3-hydrated form of (33a), and fujianmycin B (34b) as its methyl ether derivative. The structure of antibiotic PD-116740 (35), isolated from an unidentified actinomycete species (WP-4669) and possessing activity against L1210 leukaemia in vitro and HCT-8 human colon adenocarcinoma cell line [168], may be visualized as the 3-hydroxylated 8-methyl-5,6-diol derivative of (33a). All these antibiotics contain the characteristic 2-phenylnaphthalene structural feature. 

Human colon adenocarcinoma cell fine Caco-2 constitutes a popular model to study the absorption of different compounds into enterocytes and their transport through the monolayer of epithelial cells. Murota et al. [88]... 

Harper PA, Prokipcak RD, Bush LE, et al. 1991. Detection and characterization of the Ah receptor in the human colon adenocarcinoma cell line LS 180. Arch Biochem Biophys 290 27-36. 

Fantini, J., Galons, J.-P., Marvaldi, J., Cozzone, P.J., Canioni, P. (1987). Growth of a human colonic adenocarcinoma cell line (HT 29) on microcarrier beads Metabolic studies by 31phosphorus nuclear magnetic resonance spectroscopy. Int. J. Cancer 39,225-260. 

In addition to the light-harvesting property, this host-guest complex also exhibits a high photocytotoxicity. Its IC50 value, defined as the dye concentration required to kill 50% of the cells, for HT29 human colon adenocarcinoma cells is only 0.09 p,M, which is comparable with that of 113 itself formulated with Cremophor EL (0.15 p,M). The complexation with 93 (M = 2H) enhances the water solubility of 113 and therefore can prevent the use of Cremophor EL, which may cause serious hypersensitivity reactions. 

McCool D.J., Okada Y., Forstner J.F. and Forstner G.G. (1999) Roles of calreticulin and calnexin during mucin synthesis in LSI80 and HT29/A1 human colonic adenocarcinoma cells. Biochem J 341, 593-600... 

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