Sarcoma cells

The susceptibilities of some of these fluorinated purine nucleosides to the action of enzymes are now described. In contrast to the inertness of the 2 -deoxy-2 -fluoro- and 3 -deoxy-3 -fluorocytidine analogs 739, 744, and 821 towards cytidine deaminase, the adenosine compounds 867, 883, and 906 are readily deaminated - by the adenosine deaminase in erythrocytes and calf intestine, but the resulting (deaminated) inosine compounds (from 867 and 883), as well as 888, are highly resistant - to cleavage by purine nucleoside phosphorylase (to give hypoxanthine base for the first two). The reason was discussed. Both 867 and 883 can form the 5 -triphosphates, without deamination, in human erythrocytes or murine sarcoma cells in the presence of 2 -deoxycoformycin, an adenosine deaminase inhibitor, but... 

Gardner et al.75 (U.S.A.) injected ascetic Walker sarcoma cells into male albino Wistar rats and thus induced tumors in their liver. These tumors were than subjected to ECT by using an implanted... 

Gregoire V, Beauduin M, Bruniaux M, et al. Radiosensitization of mouse sarcoma cells by fludarabine (F-ara-A) or gemcitabine (dFdC), two nucleoside analogues, is not mediated by an increased induction or a repair inhibition of DNA double-strand breaks as measured by pulsed-field gel electrophoresis. Int JRadiat Biol 1998 73(5) 511-520. 

The repulsive, electrostatic forces of sialic acids contribute to the rigidity of the cell surface, as was shown hy an increase in the defor-mability of sarcoma cells after enzymic removal of sialic acid residues.424 Enzymic release of sialic acids from the zona pellucida of rabbit ovum lessens the rigidity of this cell, and spermatozoa can no longer penetrate it.425 Glycoproteins on the surface of sea-urchin eggs... 

Mege RM, Matsuzaki F, Gallin WF, Golber , Cunningham BA, Edelman GM Construction of epithelioid sheets by transfection of mouse sarcoma cells with cDNAs for chicken cell adhesion molecules. Proc Natl Acad Sci USA 1988 85 7274-7278. 

Friess, W., Zhou, W, and Groves, M.J. (1996). In vivo activity of collagen matrices containing PS1, an antineoplastic glycan, against murine sarcoma cells. Pharm. Sci., 2,121-124. 

The in vitro bioactivitivity of 29 podolactones, 15 of them natural products, against cultured Yoshida Sarcoma cells [64-65] was investigated by Hayashi s group during the period between 1975 and 1979. 

Zhou, Z., Jia, S.-F, Hung, M.-C., and Kleinerman, E. S. 2001. E1A sensitizes HER2/new-overex-pressing Ewing s sarcoma cells to topoisomerase II-targeting anticancer drugs. Cancer Res. 67 3394-3398. 

Remmelink, M., et al. 1999. In vitro influence of lectins and neoglycoconjugates on the growth of three human sarcoma cell lines. J Cancer Res Clin Oncol 125 275. 

The choice of a cell line to study MDR modulator potency was very important for future potential application in human cancer treatment. PhM (12) that were quite effective in resistant mouse lymphoma cells were only slightly active in drug-resistant human sarcoma cell line MES-SA/Dx5 [198]. The drug-sensitive human sarcoma cell line MES-SA and its multidrug-resistant counterpart MES-SA/Dx5 were applied as a model system for evaluation of MDR modulator activities. Examination performed by the flow cytometric Rhl23 accumulation test demonstrated that the well-known P-gp modulators verapamil (79) and TFP (5) reduced MDR in MES-SA/Dx5 cells. In resistant MES-SA/Dx5 cells, verapamil (79) and TFP (5) restored the drug accumulation pattern which was typical for sensitive cells. However, the effectiveness of PhM (12) was very low. The most active compounds were derivatives with an H atom at position 2 of the phenothiazine ring, followed by Cl-substituted and CF3-substituted compounds. 

Anzano MA, Roberts AB, Meyers CA, Komoriya A, Lamb LC, Smith JM, Sporn MB. Synergistic interaction of two classes of transforming growth factors from murine sarcoma cells. Cancer Research 1982, 42, 4776 -778. 

BC is a good material not only for wound treatment and other fields of veterinary medicine, but also as a scaffold material for cell cultivation in tissue engineering [156,157]. On such scaffolds the fzmb has cultivated the following cell types successfully human osteoblasts, human osteogenic sarcoma cells (SAOS-2), equine osteoblast lines and chondrocytes, and mesenchymal stem cells. 

Various types of adherent cells were grown on a coverslip, which was then laid on top of the LAPS chip. Measurements were made for acidification of (1) normal human epidermal keratinocytes stimulated by epidermal growth factor (EGP) or organic chemicals, and of (2) human uterine sarcoma cells as a response to doxorubicin and vincristine (chemotherapeutic drugs). In addition, the inhibition (by ribavirin) of the viral infection of murine fibroblastic L cells by vesicular stomatitis virus (VSV) was investigated by following the acidification rate. A limitation of these studies is the requirement for a low-buffered medium (low bicarbonate content) to achieve maximum sensitivity [847]. 

If the photoaffinity reagent has been designed well and there are a measurable number of tight binding sites for it, the protection experiment will usually succeed. Occasionally it will not for example, four polypeptides of sarcoma cells were labeled with low concentrations of 8-N3-CAMP but only three of the sites could be protected by cAMP. Presumably, the fourth site is not part of a cAMP binding protein but a different nucleotide binding site that just happens to have affinity for the reagent but not for cAMP. 

The intrinsic plasma membrane fluidity of a series of sarcoma cell lines with various degrees of adriamycin resistance was studied by ESR [91]. The results document a systematic increase in the order parameter, S, as the cells become more and more resistant. At the same time, a parallel increase in the sphingomyelin content was ob-... 

A serum-free medium supplemented with insulin, transferrin, ethanolamine and selenium (ITES) allows growth of certain hy-bridomas at 17-74% the rate found with 15% FBS (Wolpe, 1984) and Cleveland et al. (1983) devised a protein-free medium for growth of myeloma cells which, with addition of BSA at 2.5 mg/ml, forms the basis of Costar s SF-1 and SF-X supplemented media. Cloning is still very difficult in serum-free media, but feeder layers can be replaced by culture supernatants from human endothelial cells (HECS Astaldi, 1983) or Ewing s sarcoma cells (ESG Ley et al., 1980) — see 5.8.5. 

Evdokiou, A., Labrinidis, A., Bouralexis, S., Hay, S., and Findlay, D.M. (2003). Induction of cell death of human osteogenic sarcoma cells by zoledronic acid resembles anoikis. Bone 33 216-228. 

Hamed, A.I., Plaza, A., Balestrieri, M.L., Mahalel, U.A., Springuel, I.V., Oleszek, W., Pizza, C. and Piacente, S. (2006) Cardenolide glycosides from Pergularia tomen-tosa and their proapoptotic activity in Kaposi s sarcoma cells. /. Nat. Prod., 69, 1319-22. 

Several ellipticines and 7//-pyrido[4,3-c]carbazoles were examined for their effect on topoisomerase I and II from trypanosomes (Table VI) (163). The activity of 9-bromoellipticine on topoisomerase II is especially interesting since it is not a DNA intercalator. Several other bis-7//-pyrido[4,3-c]carbazoles were strongly active in this assay. As indicated in Table VII, a study of the cytotoxicity and uptake by TBL CL2 mouse sarcoma cells of several oxazolopyridocarbazoles... 

Sarcoma cells Contractile protein from undifferentiated cells 0.1-0.2 Hoffmann-Berling (1956)... 

Procedure. The assay was originally described by Passaniti (Pas-saniti et al., 1992) who studied the angiogenesis induced by bFGF and heparin. This method has been extensively used to study the angiogenic potential of Kaposi s Sarcoma cell (KS) supernatants and HIV Tat protein (Albini et al., 1994) and to test numerous an-tiangiogenic compounds (TIMP-2, IFNs, Somatostatin. ..) (Albini et al., 1994, 1999 Benelli et al., 1997 lurlaro et al., 1998). 

Albini, A., Fontanini, G., Masiello, L., Tacchetti, C., Bigini, D., Luzzi, P., Noonan, D. M. and Stetler-Stevenson, W, G. (1994). Angiogenic potential in vivo by Kaposi sarcoma cell-free supernatants and HIVl-tat product inhibition of KS-like lesions by TlMP-2. AIDS 8,1237-1244. 

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