Murine fibroblast

Andreone TL, O Connor, M, Denenberg A, Hake PW, Zingarelli B (2003) Poly(ADP-ribose) polymerase-1 regulates activation of activator protein-1 in murine fibroblasts. J Immunol 170 2113-2120... 

From strains of Sorangium cellulosum a chlorodivinylether, maracen (110), was isolated. It is active against Mycobacterium tuberculosis (IC99 < 12.5 pg/ml) and only slightly toxic against murine fibroblasts L929 (> 24 pg/ml) [89]. 

Various types of adherent cells were grown on a coverslip, which was then laid on top of the LAPS chip. Measurements were made for acidification of (1) normal human epidermal keratinocytes stimulated by epidermal growth factor (EGP) or organic chemicals, and of (2) human uterine sarcoma cells as a response to doxorubicin and vincristine (chemotherapeutic drugs). In addition, the inhibition (by ribavirin) of the viral infection of murine fibroblastic L cells by vesicular stomatitis virus (VSV) was investigated by following the acidification rate. A limitation of these studies is the requirement for a low-buffered medium (low bicarbonate content) to achieve maximum sensitivity [847]. 

Isoelectric focusing (IEF), which is usually employed for protein separation, has been used to separate mitochondria and nuclei from lysate of NR6wt cells (murine fibroblast) on a Pyrex chip [893]. 

Figure 4. Photomicrograph of 3T3 murine fibroblasts stained with Wright s-Giemsa stain a) treated with W. floribunda agglutinin (150 ng/ml) in Eagle s Minimum Essential Medium with Hanks salts for 48 hr b) control...

Yamamoto A, Honma R, Sumita M. 1998. Cytotoxicity evaluation of 43 metal salts using murine fibroblasts and osteoblastic cells. J Biomed Mater Res 39 331-340. 

Fig. 3.13 Investigation of the response of murine fibroblast cells incubated with different concentrations of NS. (a) Cell viability test conducted with the Cell-Titer-Blue assay over 4 days incubation of cells with a range of NS concentrations between 0 and 300 fM (0-1,800 NS/cell). (b) Assays of apoptosis and necrosis markers at two selected NS concentrations (10 and 300 fM), results show no difference to untreated cell controls...

The cytotoxicity of acetaminophen has been demonstrated in cultures of HeLa cells, L929 and 3T3 murine fibroblasts, chick embryo neurons, rat embryonic and skeletal muscle, peripheral blood lymphocytes, and lung and dermal cells. In addition cytotoxicity of acetaminophen has been evaluated in BF-2 fish cell line (see section on Ecotoxicology ). 

Haub, O., Drucker, B., and Goldfarb, M., Expression of the murine fibroblast growth factor-5 gene in the adult central nervous system, Proc. Natl. Acad. Sci. USA., 87, 8022, 1990. 

Fig. 5. Fluorescence pattern of murine fibroblasts after short or prolonged incubation with PrP106-126 coupled to a fluorescent dye. After one hour incubation, the fluorescent peptide is confined to the cell surface (A) while after 24 hours is internalized, the fluorescence being associated with cytoplamic organelles (B).

Entrapment/adsorption of human keratinocytes, tumor cells, and murine fibroblasts into silicone microwells Metabolic responses to bioactive agents [59]... 

The steroid derivative 1,25-dihydroxy vitamin D (vit D3), a metabolically active form of vitamin D, was shown to act as an inducer of the NGF gene in murine fibroblasts cultured in a serum-free medium (Wion et al., 1991). This effect could be detected as early as 3 h after the addition of vit D3 and resulted in an increase in both cellular mRNA and secreted NGF protein. Further experiments in the same culture system (Jehan et al.,... 

Mansukhani, A. Moscatelli, D. Talarico, D. Levytska, V. Basilico, C. A murine fibroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF and Kaposi FGF. Proc. Natl. Acad. Sci. USA, 87, 4378-4382 (1990)... 

The Absidia chitosans tested according to standard methods ASTM F813-83 and F619-79 with murine fibroblasts were found to be biocompatible in terms of cytotoxicity and lactate dehydrogenase activity, in agreement with Chung et al. [31]. By contrast, for solid-state fermentation, the chitosan production was higher (6.12 g/kg) but the MW was only 6.4 kDa [32]. 

The cloned human receptor was stably expressed in murine fibroblast cells (L-MCTK )cells whereas the Ala-242 mutant receptor was expressed in COS-7 cells. 

Fig. 10. Transfection experiments performed with dendrimers 36-[G ] and 37-[G ] in the presence of 10% PCS (serum). NIH 3T3 ceii iine (murine fibroblast). 2 microg of pCMVLuc/well. Expression stopped 24 h later...

No genotoxic effects of the compound norbixin were observed in murine fibroblasts (Kovary et al. 2001). 

Several chemokines were originally identified as IFN-y-induced genes. The CXCLlO/IP-10 gene has a Statl-binding site in the promoter that is transacti-vated by Statl in both human and murine fibroblasts (12,13). In Statl-deficient... 

There is, however, some evidence that UDPGlc can be a direct donor in other systems (rabbit reticulocyte and human erythrocyte — Parodi and Martin Barrientos, 1977 Martin Barrientos and Parodi, 1977 murine fibroblasts — Robbins, Krag and Lui, 1977) and further information upon this possible variation would be desirable. 

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