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Blots

Sous-vide processing Southern blot Southern blotting... [Pg.917]

Fig. 1. Southern blot analysis of DNA showing (a) step 1, an agarose gel containing separated restriction fragments of DNA, denoted by (—), which is immersed in NaOH to denature the double-stranded stmcture of DNA, and then transferred by capillary flow to a nitrocellulose filter. In step 2, the bound DNA is allowed to hybridize to a labeled nucleic acid probe, and the unbound probe is washed off In step 3, the filter is placed into contact with x-ray film resulting in (b) bands of exposure on the film which are detected after development and correspond to regions where the restriction fragment is... Fig. 1. Southern blot analysis of DNA showing (a) step 1, an agarose gel containing separated restriction fragments of DNA, denoted by (—), which is immersed in NaOH to denature the double-stranded stmcture of DNA, and then transferred by capillary flow to a nitrocellulose filter. In step 2, the bound DNA is allowed to hybridize to a labeled nucleic acid probe, and the unbound probe is washed off In step 3, the filter is placed into contact with x-ray film resulting in (b) bands of exposure on the film which are detected after development and correspond to regions where the restriction fragment is...
The emission yield from the horseradish peroxidase (HRP)-catalyzed luminol oxidations can be kicreased as much as a thousandfold upon addition of substituted phenols, eg, -iodophenol, -phenylphenol, or 6-hydroxybenzothiazole (119). Enhanced chemiluminescence, as this phenomenon is termed, has been the basis for several very sensitive immunometric assays that surpass the sensitivity of radioassay (120) techniques and has also been developed for detection of nucleic acid probes ia dot-slot. Southern, and Northern blot formats (121). [Pg.268]

Chemiluminescence and bioluminescence are also used in immunoassays to detect conventional enzyme labels (eg, alkaline phosphatase, P-galactosidase, glucose oxidase, glucose 6-phosphate dehydrogenase, horseradish peroxidase, microperoxidase, xanthine oxidase). The enhanced chemiluminescence assay for horseradish peroxidase (luminol-peroxide-4-iodophenol detection reagent) and various chemiluminescence adamantyl 1,2-dioxetane aryl phosphate substrates, eg, (11) and (15) for alkaline phosphatase labels are in routine use in immunoassay analyzers and in Western blotting kits (261—266). [Pg.275]

The use of agarose as an electrophoretic method is widespread (32—35). An example of its use is in the evaluation and typing of DNA both in forensics (see Forensic chemistry) and to study heritable diseases (36). Agarose electrophoresis is combined with other analytical tools such as Southern blotting, polymerase chain reaction, and fluorescence. The advantages of agarose electrophoresis are that it requires no additives or cross-linkers for polymerization, it is not hazardous, low concentration gels are relatively sturdy, it is inexpensive, and it can be combined with many other analytical methods. [Pg.182]

Most sample components analyzed with electrophoretic techniques are invisible to the naked eye. Thus methods have been developed to visualize and quantify separated compounds. These techniques most commonly involve chemically fixing and then staining the compounds in the gel. Other detection techniques can sometimes yield more information, such as detection using antibodies to specific compounds, which gives positive identification of a sample component either by immunoelectrophoretic or blotting techniques, or enhanced detection by combining two different electrophoresis methods in two-dimensional electrophoretic techniques. [Pg.183]

Eor example, the technique of Southern blotting was developed (68) for use with agarose gel electrophoresis of DNA fragments. Southern blots are designed to detect specific sequences of DNA. After electrophoresis is complete, the DNA is denatured and the single stranded DNA transferred to the specially prepared nitrocellulose paper. The nitrocellulose is then incubated with radioactive RNA or DNA complementary to those DNA sequences of interest. After the nitrocellulose has been sufftciendy incubated with the radioactive complementary DNA, autoradiography is used to identify the fragments of interest. [Pg.184]

The northern blotting technique is similar to the Southern blotting technique with the exception that northern blots detect specific sequences of RNA, not DNA. [Pg.184]

The technique of immun obi otting, often referred to as western blotting, is frequendy used for a variety of appHcations where protein concentrations... [Pg.184]

Blotting techniques may be used in a variety and combination of electrophoretic systems which makes their use widespread and convenient when protein concentrations are minimal and agarose or polyacrylamide is the matrix choice. [Pg.185]

B. A. Baldo and E. R. Tovey, Protein Blotting. Methodology, Kesearch, and Diagnostic Applications, Karger, Swit2edand, 1989. [Pg.186]

Layer of sand and gravel to regulate flow of water, separated by metallic gauge and blotting paper. [Pg.267]

A piece of sodium metal stored under kerosene m a metal container is removed from ajar and blotted with dry napkin or filter paper With a sharp knife, the layer of oxides IS removed until a shiny surface appears The removed layer is then destroyed carefully by adding very small pieces (not larger than 0 5 cm) to precooled 200 mL of methanol or ethanol... [Pg.1024]

Identifying Specific DNA Sequences by Southern Blotting (Southern Hybridization)... [Pg.410]

Transfer (blot) gel to nitrocellulose filter using Soudiern blot technique... [Pg.411]

AusUfis-rdhre, /. outlet tube or pipe, discharge pipe, -ventil, n. escape valve, delivery valve. Auslauf, m. outlet, mouth running out outflow, discharge leaking, leak outrun, outspread start projection, auslaufen, v.i. run out leak out, leak blot (of colors) run, bleed project wear out, play out stop running set out. [Pg.48]

Fleck, m. spot speck, stain, blot, flaw, patch ... [Pg.157]


See other pages where Blots is mentioned: [Pg.812]    [Pg.921]    [Pg.513]    [Pg.229]    [Pg.229]    [Pg.231]    [Pg.232]    [Pg.241]    [Pg.244]    [Pg.275]    [Pg.5]    [Pg.440]    [Pg.340]    [Pg.526]    [Pg.184]    [Pg.185]    [Pg.86]    [Pg.336]    [Pg.407]    [Pg.410]    [Pg.410]    [Pg.410]    [Pg.410]    [Pg.197]    [Pg.356]    [Pg.7]    [Pg.48]    [Pg.60]    [Pg.62]    [Pg.113]    [Pg.157]    [Pg.158]    [Pg.158]    [Pg.246]   
See also in sourсe #XX -- [ Pg.514 ]




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Acyl-CoA hydrolases northern blotting

Affinity Blotting

Antibodies Western blot

Antibodies Western blotting

Antibody blotting

Assay blotting

Assay protein, Western blotting

Autoradiography of Gels and Blots

Avidin blotting

Blot analysis

Blot membranes

Blot membranes blocking

Blot membranes protein binding

Blot overlay assay

Blot transfer techniques

Blots Blotting

Blots Blotting

Blots gold labeling

Blotted protein

Blotting

Blotting

Blotting Blue gels

Blotting Immunoblotting

Blotting Northern

Blotting description

Blotting diagram

Blotting gold labeling

Blotting hybridization

Blotting immunostaining

Blotting membranes

Blotting methods

Blotting of proteins

Blotting techniques

Blotting transfer buffers

Capillary blots

Chemiluminescence Detection on Blotting Membranes

Chemiluminescence, enhanced Western blot

Colony blot hybridization

DNA Southern blot

DNA blots

Deoxyribonucleic acid blotting

Detection blot overlay assay

Detection of Glycoproteins on Blots

Detection of Proteins on Blot Membranes

Dot blot

Dot blot assay

Dot blot hybridization

Dot blot technique

Dot blot test

Dot blotting

Dot-blot analysis

Double replica blotting

Electrophoresis Northern blotting

Electrophoresis Southern blotting

Electrophoresis blotting

Enhanced chemiluminescence Western blotting

Enzyme immunoassays and related techniques with protein blots

Experiment 18 Western Blot to Identify an Antigen

Far-Western blotting

Filter paper blotting

Fusion western blot

Gel blots

General Carbohydrate Detection on Western Blots

General immunostain of blotted proteins

Genomic Southern blot

Genomic Southern blot hybridization

Genomic blots

Glycoproteins on blots

Hybridization Southern blot

Immuno-dot blot analysis

Immunochemical techniques Western blotting

In Western blotting

Lectins protein-blot analysis

Ligand blotting

North Western blotting

Northern and Southern Blot Analyses

Northern blot analysis

Northern blot hybridization

Northern blot transfer procedure

Northern blots

Northern blotting analysis

Northern blotting principles

Northwestern blot

Nucleic acid hybridization Northern blotting

Nucleic acid hybridization Southern blotting

Nucleic acids blotting

Peroxisome Western blot

Pocket blotting

Polymer blotting method

Progression blot analysis

Protein and Nucleic Acid Blotting

Protein blots

Protein-blot analysis

Protein-blot analysis of glycoproteins

Proteins blotting

Proteomics Western blots

Purification of antibodies on protein blots

Qualitative analysis Western blotting

RNA blots

Reverse blot assays

Ribonucleic acid blotting

Semi-dry blotting

Serum Glycoproteins by SDS-PAGE and Western Blotting

Slot blots

Slot blotting

Southern blot

Southern blot analyses

Southern blot analysis specific

Southern blot procedure

Southern blot protocol

Southern blot technique

Southern blot transfer procedure

Southern blots hybrid selection

Southern blots probe labeling

Southern blotting

Southern blotting principle

Southern blotting probe, preparation

Southwestern blotting

Staining blots

The Northern Blot

The Southern Blot

The Western Blot

Thin-layer chromatography-blotting

Transfer blotting

Transfer of Nucleic Acids (Southern and Northern Blot)

Two-dimensional Northwestern blotting

Western blot

Western blot analysis

Western blot antibodies specificity determination

Western blot assay

Western blot blotting protocol

Western blot experiments

Western blot fractions

Western blot immunodetection

Western blot method

Western blot techniques

Western blot transfer procedure

Western blots hepatocytes

Western blots, ginsenosides

Western blotting

Western blotting analysis

Western blotting materials

Western blotting method

Western blotting primary antibodies

Western blotting technique

Western blotting technique sections

Western blotting/enzyme-linked

Western blotting/enzyme-linked ELISAs)

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