Pocket blotting

Pocket blotting, centrifugal transfer and vacuum or positive pressure transfer... 

Pocket blotting (Cuny et al., 1991) is a simple, low cost alternative to vacuum blotting and does not require special equipment. It is slightly slower, but demands less attention and allows a large number of gels to be blotted simultaneously (Table 9.9C). 

Immerse the test package in a container containing coloured water (15-25°C) and place the container in the vacuum chamber. Apply the appropriate vacuum of 33 kPa (250 mm of mercury) for strip packages or 24 kPa (180 mm of mercury) for blister packages, for 30 s. Restore atmospheric pressure and remove the container from the vacuum chamber. Remove the test package from the container and blot off the excess water. Examine the package for ingress of water into the pockets. 

Mi trail leur (Fire-arms.) A French form of batteiy-gun which is loaded at the breech, by a blot k containing pockets for a number of cartridges. These are fired consecutively or in a volley. Charged plates are kept in reserve. 

For the characterization of glycoproteins with lectins, the sample is run on an SDS gel with an approximate 10-cm-wide pocket and then blotted. The blot (on PVDF membrane) is stained on protein and the approximately 10-cm-long protein band is cut crosswise into approximately 20 narrow strips. After blocking (e.g., with 2% polyvinylpyrrolidon 360 in 50 mM Tris-Cl pH 7.5 and 0.5 M NaCl), the strips are incubated in twos with a labeled lectin (Table 9.1) with or without inhibiting sugar, and are then developed. 

Drain the blot(s) on a piece of absorbent paper and wrap them in a fresh piece of Saran Wrap try to avoid air pockets. 

Ballpoint pen. For ballpoint pen ink stains on your dress-shirt pockets, rinse the stain with glycerin, not water, at your earliest opportunity. Let the fabric soak for at least 10 minutes, up to an hour, then apply detergent mixed with water and add two or three drops of ammonia. Let the ammonia soak in for an hour, blotting occasionally. Rinse thoroughly. If the stain remains, dab on a solution of bleach and water, but only if the garment is white or colorfast. 

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