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Western blot method

The Western blot method is often used in the analysis of host cell impurities. It can be used to identify a recurring impurity. O Keefe et al. used a Western blot to identify an E. coli protein impurity in the preparation of the recombinant fibroblast growth factor (aFGF).29 By using specific antisera to the E. coli host cell proteins, they were able to isolate the impurity and determine its N-terminus amino acid sequence to confirm its identity. Antibodies could be used to determine the concentration of this impurity in sample preparations. [Pg.298]

Okamura, H., Sigal, C. T., Alland, L., and Resh, M. D. (1995) Rapid high-resolution western blotting. Methods Enzymol. 254, 535-550. [Pg.130]

Shan, S.J. Tanaka, H. Shoyama, Y. Western blotting method for the iimnunostaining detection of glucuronides of glycyrrhetic acid using anti-glycyrrhizin monoclonal antibody. Biol. Pharm. Bull. 1999, 22, 221. [Pg.2249]

See other pages where Western blot method is mentioned: [Pg.148]    [Pg.155]    [Pg.155]    [Pg.36]    [Pg.135]    [Pg.462]    [Pg.1630]    [Pg.1632]    [Pg.47]    [Pg.54]    [Pg.258]    [Pg.36]    [Pg.294]    [Pg.299]    [Pg.299]    [Pg.302]    [Pg.244]    [Pg.2247]    [Pg.2248]    [Pg.25]    [Pg.1558]    [Pg.1560]    [Pg.103]    [Pg.105]   
See also in sourсe #XX -- [ Pg.234 , Pg.299 , Pg.302 ]



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