Phosphate dehydrogenase

C.F., Oswald, A. S., Shen, T.Y., Kinter, M., Tang, G.Z., and Zeller, K. Rat liver insulin mediator which stimulates pyruvate dehydrogenase phosphate contains galactosamine and D-chiroinositol. Biochem. Biophys. Res. Commun., 1988, 151, 1416-1426. 

Pyruvate dehydrogenase phosphate phosphatase is stimulated by Ca. In muscles, the Ca + concentration increases dramatically during muscle contraction. How would the elevated Ca + concentration affect the rate of glycolysis and the citric acid cycle ... 

A cell is deficient in pyruvate dehydrogenase phosphate phosphatase. How would such a deficiency affect cellular metabolism ... 

ATP is an important source of energy for muscle contraction. Pyruvate dehydrogenase phosphate phosphatase is activated by calcium ion, which increases greatly in concentration during exercise. Why is activation of the phosphatase consistent veith the metabolic requirements of muscle during contraction ... 

Pyruvic and lactic acids Pyruvate decarboxylase EC4.1.1.1) Pyruvate dehydrogenase phosphate phosphatase (EC 1.2.3.3), Dihydrolipoyl dehydrogenase (ECl.6.4.3) 15.4... 

Clinical Analysis. A wide range of clinically important substances can be detected and quantitated using chemiluminescence or bioluminescence methods. Coupled enzyme assay protocols permit the measurement of kinase, dehydrogenase, and oxidases or the substrates of these enzymes as exemplified by reactions of glucose, creatine phosphate, and bile acid in the following ... 

Chemiluminescence and bioluminescence are also used in immunoassays to detect conventional enzyme labels (eg, alkaline phosphatase, P-galactosidase, glucose oxidase, glucose 6-phosphate dehydrogenase, horseradish peroxidase, microperoxidase, xanthine oxidase). The enhanced chemiluminescence assay for horseradish peroxidase (luminol-peroxide-4-iodophenol detection reagent) and various chemiluminescence adamantyl 1,2-dioxetane aryl phosphate substrates, eg, (11) and (15) for alkaline phosphatase labels are in routine use in immunoassay analyzers and in Western blotting kits (261—266). 

Two or more linked enzyme reactions can lead to a change in the concentration of NADH or NADPH that is equivalent to the concentration of the original analyte. The reference glucose measurement using hexokinase [9001-51-8] and glucose-6-phosphate dehydrogenase [9001-40-5] is an example ... 

Enzymes, measured in clinical laboratories, for which kits are available include y-glutamyl transferase (GGT), alanine transferase [9000-86-6] (ALT), aldolase, a-amylase [9000-90-2] aspartate aminotransferase [9000-97-9], creatine kinase and its isoenzymes, galactose-l-phosphate uridyl transferase, Hpase, malate dehydrogenase [9001 -64-3], 5 -nucleotidase, phosphohexose isomerase, and pymvate kinase [9001-59-6]. One example is the measurement of aspartate aminotransferase, where the reaction is followed by monitoring the loss of NADH ... 

In this biosensor a quartz radio crystal is functionalized with the enzyme glucose-6-phosphate dehydrogenase. As shown in Figure 3, a thin film of Pmssian blue [14038-43-8] C gN gFe, is then coated onto the crystal. 

Fig. 4. Schematic of a multisequence biosensor in which the target glucose is first converted to glucose-6-phosphate, G6P, in the test solution by hexokinase. G6P then reacts selectively with glucose-6-phosphate dehydrogenase immobilized on the quartz crystal surface. Electrons released in the reaction then chemically reduce the Pmssian blue film (see Fig. 3), forcing an uptake of potassium ions. The resulting mass increase is manifested as a...

Acetoin dehydrogenase [from beef liver acetoin NAD oxidoreductase] [9028-49-3] Mr 76000, [EC 1.1.1.5]. Purified via the acetone cake then Ca-phosphate gel filtration (unabsorbed), lyophilised and then fractionated through a DEAE-22 cellulose column. The Km for diacetyl in 40pM and for... 

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