Alkaline phosphatase as label

As a first model system goat-IgG (gtIgG) was chosen as an analyte The use of alkaline phosphatase as label required a change the pH between the immunoassay (pH 8) and the electrode reaction (pH 6.5). In order to reduce background signal activity determination was performed with 1 iM PAPP. In 0.1 M MES buffer the electrode sensitivity towards PAPP is approximately 10 % of the respective response in the presence of the same concentration of PAP At the same time, the sensitivity towards PAPP in phosphate buffer is 200 times lower In comparison to PAP sensitivity. This suggests that the enzyme membrane causes the hydrolysis of PAPP to PAP. Obviously, phosphate ions inhibit this process. 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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