Peroxisome Western blot

A representative Western blot is shown in Figure 1. In all three membrane fractions only a protein that migrates with an apparent mass of 88kDa was reproducibly labelled by DNP-etomoxiryl-CoA when quantitative CPT inhibition had been achieved. A smaller protein was sometimes labelled in peroxisomes, but its extent of labelling was very variable and not proportional to CPT activity. While for mitochondria and peroxisomes the labelling of the 88kDa protein was as expected from the studies the result... 

Western Blot Analysis of the COT Proteins in Rat Liver Peroxisomes... 

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