Enzyme immunoassays and related techniques with protein blots

Enzyme immunoassays and related techniques with protein blots 

Protein blots can be used (i) to identify various constituents of a mixture and to establish their relationship(s) by EIH (epitope mapping) (ii) to localize these constituents by the sensitive immunostain after their haptenation and subsequent reaction with labeled anti-hapten antibodies and, (iii) to elute antibodies immobilized to a certain protein band ( poor man s monoclonals ). 

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