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Glycoproteins on blots

Bayer, E.A., Ben-Hur, H., and Wilchek, M. (1987a) Enzyme-based detection of glycoproteins on blot transfers using avidin-biotin technology. Anal. Biochem. 161, 123-131. [Pg.1046]

Protein-labeled colloidal gold probes suit as well as for immuno-histochemistry in electron microscopy as for detection of antigens or glycoproteins on blots. There are several protocols for enhancement of electron density and visible color resulting in higher sensitivity. [Pg.141]

Haselbeck, A. and Hosel, W. (1990) Description and application of an immunological detection system for analyzing glycoproteins on blots. Glycoconjugate J. 7, 63-74. [Pg.94]

On completion of electrophoresis, proceed with transfer of glycoproteins onto a polyvinylidene difluoride (PVDF) membrane. Remember to discard the upper stacking gel and to wet the PVDF membrane in methanol before use. Wet transfer may be done according to the procedure described in Chapter 8 For semidry blotting, follow the procedure described in Chapter 20. [Pg.89]


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See also in sourсe #XX -- [ Pg.206 , Pg.208 ]




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