Polymer blotting method

Nishimura and co-workers have developed the catch-and-release strategy between solid-phase and water-soluble polymer supports, the so called pol)uner blotting method , which allows for the rapid and efficient synthesis of glycopeptides [76]. The method involves (i) conven-... 

The transport of many compounds takes place through interstices of polymer chains filled with aqueous medium [52], In such cases, the rate of mass transport is directly proportional to the degree of hydration of the membranes [53]. The most widely accepted method for determining the hydration of membranes is to equilibrate the membranes in water or buffer and weigh these membranes after blotting [54], In a newer method, the matrices to be studied are placed on a sintered glass funnel which is attached to a capillary filled with water. The absorption of water results in the movement of the capillary front [55],... 

The primary components of LCM technology are (1) visualization of the cells of interest through microscopy, (2) transfer of near-infrared laser energy pulses to a thermolabile polymer with formation of a polymer-cell composite, and (3) removal of the polymer from the tissue surface, which shears the embedded cells of interest away from the heterogeneous tissue section (18,19). Extraction buffers applied to the polymer film solubilize the cells, liberating the molecules of interest. The DNA, RNA, or protein from the microdissected cells may be analyzed by any method with appropriate sensitivity (20,21,22,23,24). Protein extracted from microdissected cells may be used for mass spectrometric analysis, applied to reverse phase protein microarrays, or used for western blot analysis (25,26). 

Polymers can also be swollen with the solution of an oxidant, blotted with tissue or filter paper and dried, retaining the oxidant as a filler in its bulk. The blend is obtained by exposing the polymer containing the oxidant to vapors of the heterocyclic monomer in a closed chamber. Blends were prepared using this method and combining polypyrrole with cellulose [28], with poly (ethylene terephthalate) [29] or with Nylon [30]. 

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