Immunochemical techniques Western blotting

Western blotting is one of the most widely used immunochemical techniques in modern biochemistry. In general, it is used to detect the presence of a particular protein antigen in an impure mixture. Western blots can provide information that is (1) qualitative Is the protein present (2) quantitative How much of the protein is present and (3) structural What is the size of the protein The general protocol for Western blotting is as follows (see Fig. 18-1) ... 

The western blot, also known as the immunoblot, is used to identify and relatively quantify the amount of a specific protein within a sample containing multiple proteins, such as what is obtained following cell lysis. An immunochemical assay is used for protein identification. The technique is often used to detect the downstream product of specific gene expression. Due to high sensitivity, efficiency, and convenience, the western blot has been widely used by researchers through the world. 

Immunochemical methods are highly sensitive techniques for the detection and identification of protein targets by antigen-antibody specific reactions. Radial immunodilfusion and western blotting are among the most used techniques for the quantitative estimation of processed food and bioproduct proteins based on their immunoreactivity with specific antibodies. 

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