Detection reagents for

Figure 7.21 Dendrimers that are fluorescently labeled as well as biotinylated create enhanced detection reagents for use in (strept)avidin-biotin-based assays. Large complexes containing multiple fluorescent dendrimers can bind to antigens and form a highly sensitive detection system that exceeds the detection capability of fluorescently labeled antibodies.

Colloidal gold-labeled (strept)avidin can be used as highly sensitive detection reagents for microscopy techniques (Cubie and Norval, 1989) (Chapter 24). Finally, cytotoxic substances coupled to (strept)avidin can be used to direct cell-killing activity toward a tumor-cell-bound, biotinylated monoclonal antibody (or other targeting molecule) for cancer therapy (Hashimoto et al, 1984) (Chapter 21). 

ZytoVision ZytoDot2C GISH Implementation Kit contains all necessary BISH detection reagents for the detection of DIG-and DNP-labeled DNA probes on formalin-fixed tissue sections. The probes can be purchased from ZytoVision or other vendors. 

Dako DuoCISH Kit contains HRP- and AP-based immunological detection reagents for Texas Red- and FITC-labeled probes. This is an ideal kit for manual dual-color BISH assay detection. 

A versatile detection reagent for lipids is iodine vapor. The dry plate is placed into a chamber containing one or two Petri dishes with crystals of solid iodine. Lipid-containing spots are colored yellow to brownish after few minutes. The color disappears quickly in contact with air. 

Drain off any excess wash buffer from the membrane, and place it in the detection reagent for 1 min It is important that the membrane be completely covered by the reagent... 

O-Phthaldialdehyde (OPA) is an amine detection reagent that reacts in the presence of 2-mercaptoethanol to generate a fluorescent product (for preparation, see Section 4.1, 2-mercaptoethanol) (Fig. 91). The resultant fluorophore has an excitation wavelength of 360 nm and an emission point at 455 nm. OPA can be used as a sensitive detection reagent for the HPLC separation of amino acids, peptides, and proteins (Fried et al., 1985). It is also possible to measure the amine content in proteins and other molecules using a test tube or microplate format assay with OPA. Detection limits are typically in the microgram per milliliter range for proteins. 

Dipping solution I alone is a sensitive detection reagent for phosphate esters. Combination with dipping solution II does increase the sensitivity limit for organic... 

Short ECL protocol Block blot (1 to 2 h), incubate first antibody (1 h), wash well, incubate second (peroxidase-marked) antibody, wash well, detect reagent for 1 minute, wrap blot in cellophane, and incubate with film for 30 seconds to 30 minutes. 

Blocking reagents Samples are blocked after hybridization but before staining with antibodies or other detection reagents. For this we use a 0.5%... 

Detection reagents for biochemical reactions Indole reagent (according to Kovacs) (Recipe 30) Composition... 

Scorilas A, Diamandis EP (2000) Polyvinylamine-streptavidin complexes labeled with a europium chelator a universal detection reagent for solid-phase time resolved fluorometric applications. Clin Biochem 33 345-350... 

Qin QP, Lovgren T, Pettersson K (2001) Development of highly fluorescent detection reagents for the constmction of ultrasensitive immunoassays. Anal Chem 73 1521-1529... 

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