Blotting diagram

Diagram of a blotting experiment. From J. Watson, Molecular Biology of the Gene, 4th ed. (1987), Benjamin/Cum-mings Publishing Company (Redwood City, CA). 

Figure 1-5. Diagram of the fragile X mental retardation (FAIR ) gene with restriction map and FMR1 probes used for diagnostic Southern blots. The circle indicates the CpG island, and the box represents the first exon. The dark region shows the location of triplet repeats.

Fig. 7. Southern blotting. DNA fragments that have been separated by gel electrophoresis are transferred to nylon membrane, such as Nytran, as shown in the diagram and as described in the text.

Figure 11.3 Schematic diagram ofthe methods for estimating the contribution of each transporter to the overall hepatic uptake [250] (a) using reference compounds (b) using the relative expression levels estimated from Western blot analysis and (c) using transporter-specific inhibitors. The details are described in the Section 11.5.2.

A EXPERIMENTAL FIGURE 9-26 Southern blot technique can detect a specific DNA fragment in a complex mixture of restriction fragments. The diagram depicts three different restriction fragments in the gel, but the procedure can be applied to a mixture of millions of DNA fragments. Only fragments that... 

Fig. 15.2 Schematic diagram of an in vitro perfusion system for laminar shear stress conditions. Flow chamber contains the biomaterial with cultivated cells (e.g. HUVECs). Perfusion with full blood, isolated leukocytes, platelets or cell culture media. Rolling pump applies various volume rates. After perfusion, the perfused cells on the biomaterial (e.g. proliferation, vitality, PCR, Western blotting) and the supernatant (e.g. flow cytometry, ELISA) should be analyzed...

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