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Lectins protein-blot analysis

Protein-Blot Analysis of Glycoproteins and Lectin Overlays... [Pg.323]

Western blotting has become an important, modern technique for analysis and characterization of proteins. The procedure consists of, first, the electrophoretic transfer (blotting) of proteins from polyacrylamide gels to synthetic membranes. The transferred blots are then probed using immunological detection methods to identify proteins of specific structure and/or function. In this experiment, bovine serum will be fractionated by SDS-PAGE and the proteins blotted onto a nitrocellulose membrane. Serum glycoproteins will be identified by their specific interaction with the lectin concanavalin A. [Pg.321]

See other pages where Lectins protein-blot analysis is mentioned: [Pg.434]    [Pg.437]    [Pg.369]    [Pg.731]    [Pg.2063]    [Pg.266]    [Pg.263]    [Pg.119]    [Pg.224]   
See also in sourсe #XX -- [ Pg.3 , Pg.323 , Pg.324 , Pg.325 , Pg.326 , Pg.327 , Pg.328 , Pg.329 , Pg.330 ]



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