Qualitative analysis Western blotting

The amount of immobilized protein can be determined by the mass balance between initial and final solutions [56], A combined qualitative method merged from the classical in-situ detection of enzyme activity and western blot analysis can be applied to determine the enzyme spatial distribution through the membrane thickness and along the membrane module and its activity after the immobilization [57-59]. 

The presence of the enzyme-g3p fusion protein should also be detected by western blot analysis with an anti-g3P and/or an anti-enzyme antibody. With anti-g3p detection, the relative intensities of the fusion and the free g3p proteins should be in qualitative agreement with the displayed level evaluated by the ratio between the enzyme and the phage concentration. 

Fig. 8. Array-based qualitative analysis of immobilisation efficiencies. Flere an array of 48 diverse BCCP-tagged human proteins was printed in triplicate, with 4 replica arrays per slide. Western blot-style analysis, probing with an anti-c-myc antibody, reveals that all proteins are immobilised as expected apparent gaps on the array are due to the printing pattern used here.

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