Antigens retrieval

Buchwalow and W. Bocker, Immunohistochemistry Basics and Methods, DOI 10.1007/978 3 642 04609 4 6, Springer Verlag Berlin Heidelberg 2010 [Pg.47]

Pre-warm two containers of TBS to 37 C (if using a water-bath this will take only 15-30 min, if using an oven leave overnight). A 50 ml Coplin jar can be used for small numbers of slides, a 400 ml trough for larger numbers. [Pg.396]

Immediately before use make the enzyme solution and transfer the warmed slides quicldy into it, incubate for 10-20 min with occasional agitation. [Pg.396]

When the incubation time is finished quickly transfer the slides to running tap-water and wash them well in this for 5 min to remove all traces of the enzyme. Discard the enzjmie solution. [Pg.396]

The introduction of HTAR methods has had a major impact on immunocyto-chemistiy, allowing reliable staining of a plethora of epitopes previously not or barely demonstrable in formalin-fixed paraffin processed material. [Pg.397]

Microwave oven (with turntable and digital timer) r [Pg.397]

RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES AND ITS APPLICATION... [Pg.1]

STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH... [Pg.3]

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics, Edited by Shan-Rong Shi and Clive R. Taylor Copyright 2010 John Wiley Sons, Inc. [Pg.3]

TABLE 1.1 Test Battery Suggested for Screening an Optimal Antigen Retrieval Protocol... [Pg.4]

Sample Frozen FFPE Section with Antigen Retrieval ... [Pg.10]

Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 1991 39 741-748. [Pg.20]

Taylor CR. Standardization in immunohistochemistry the role of antigen retrieval in molecular morphology. Biotech. Histochem. 2006 81 3-12. [Pg.20]

Shi SR, Cote RJ, Yang C, et al. Development of an optimal protocol for antigen retrieval a test battery approach exemplified with reference to the staining of retinoblastoma protein (pRB) in formalin-fixed paraffin sections. J. Pathol. 1996 179 347-352. [Pg.21]

Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry past, present, and future./. Histochem. Cytochem. 1997 45 327-343. [Pg.21]

Shi S-R, Cote RJ, Chaiwun B, et al. Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 1998 6 89-96. [Pg.21]

Shi S-R, Cote RJ, Taylor CR. Standardization and further development of antigen retrieval immunohistochemistry strategies and future goals. I. Histotechnol. 1999 22 177-192. [Pg.21]

Rocken C, Roessner A. An evaluation of antigen retrieval procedures for immu-noelectron microscopic classification of amyloid deposits. J. Histochem. Cytochem. 1999 47 1385-1394. [Pg.21]

Yano S, Kashima K, Daa T, et al. An antigen retrieval method using an alkaline solution allows immunoelectron microscopic identification of secretory granules in conventional epoxy-embedded tissue sections. /. Histochem. Cytochem. 2003 51 199-204. [Pg.21]

Saito N, Konishi K, Takeda H, et al. Antigen retrieval trial for post-embedding immunoelectron microscopy by heating with several unmasking solutions. J. Histochem. Cytochem. 2003 51 989-994. [Pg.21]

Naito I, Ninomiya Y, Nomura S. Immunohistochemical diagnosis of Alport s syndrome in paraffin-embedded renal sections antigen retrieval with autoclave heating. Med. Electron Microsc. 2003 36 1-7. [Pg.21]

Ge S, Crooks GM, McNamara G, et al. Fluorescent immunohistochemistry and in situ hybridization analysis of mouse pancreas using low-power antigen-retrieval technique. /. Histochem. Cytochem. 2006 54 843-847. [Pg.22]

Slater MD, Murphy CR. Co-expression of interleukin-6 and human growth hormone in apparently normal prostate biopsies that ultimately progress to prostate cancer using low pH, high temperature antigen retrieval. /. Mol. Histol. 2006 37 37-41. [Pg.22]

Namimatsu S, Ghazizadeh M, Sugisaki Y. Reversing the effects of formalin fixation with citraconic anhydride and heat a universal antigen retrieval method. J. Histochem. Cytochem. 2005 53 3-11. [Pg.22]

Wilson E, Jackson S, Cruwys S, et al. An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues. J. Immunol. Methods 2007 322 137-142. [Pg.22]

Pileri SA, Roncador G, Ceccarelli C, et al. Antigen retrieval techniques in immunohistochemistry comparison of different methods. I. Pathol. 1997 183 116-123. [Pg.22]

Evers P, Uylings HB. An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative. I. Neurosci. Methods 1997 72 197-207. [Pg.23]

Evers P, Uylings HB, Suurmeijer AJ. Antigen retrieval in formaldehyde-fixed human brain tissue. Methods 1998 15 133-140. [Pg.23]

Jiao Y, Sun Z, Lee T, et al. A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections./. Neurosci. Methods 1999 93 149-162. [Pg.23]

Vassallo J, Pinto GA, Alvarenga JM, et al. Comparison of immunoexpression of 2 antibodies for estrogen receptors (1D5 and 6F11) in breast carcinomas using different antigen retrieval and detection methods. Appl. Immunohistochem. Mol. Morphol. 2004 12 177-182. [Pg.23]

Kan RK, Pleva CM, Hamilton TA, et al. Immunolocalization of MAP-2 in routinely formalin-fixed, paraffin-embedded guinea pig brain sections using micro-wave irradiation a comparison of different combinations of antibody clones and antigen retrieval buffer solutions. Micros. Microanal. 2005 11 175-180. [Pg.23]

Mighell AJ, Robinson PA, Hume WJ. Patterns of immunoreactivity to an anti-fibronectin polyclonal antibody in formalin-fixed, paraffin-embedded oral tissues are dependent on methods of antigen retrieval. J. Histochem. Cytochem. 1995 43 1107-1114. [Pg.24]

Hann CR, Springett MJ, Johnson DH. Antigen retrieval of basement membrane proteins from archival eye tissues. J. Histochem. Cytochem. 2001 49 475-482. [Pg.24]

EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION... [Pg.25]

TABLE 2.1 Antigen Retrieval Used for Non-Formalin-Fixed Cytological Slides... [Pg.28]

Reference Sample Condition Antigen Retrieval IHC (IP) Results... [Pg.28]

Shi S-R, Gu J,Taylor CR. Antigen Retrieval Techniques Immunohistochemistry and Molecular Morphology, 1st edition. Natick, M A Eaton, 2000. [Pg.41]

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