STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE

STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE 

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics, Edited by Shan-Rong Shi and Clive R. Taylor Copyright 2010 John Wiley Sons, Inc. 

Leong postulated that internal controls were required to optimize the variable inhuences resulting from aspects of tissue preparation and factors intrinsic to the staining method. Various controls have been employed, includ- 

Ten years ago, we conducted an experiment using AR-IHC on FFPE tissues fixed in formalin for different periods ranging from 4 h to 30 days to explore 

Nevertheless, caution must be taken to avoid any false IHC negative or false positive results after AR treatment. It should be emphasized that additional biochemical assays other than IHC need to be adopted in order to validate the AR-IHC results whenever necessary. Otherwise, as pointed out by Wick and Mills, there is a real risk that artifacts may become facts.  

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Shi S-R, Cote RJ, Chaiwun B, et al. Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 1998 6 89-96. 

Figure 5.3 Diagram depicts the further-designed studies to test our hypothesis with respect to standardization of immunohistochemistry based on the antigen retrieval technique exemplified in a multiple direction to draw a conclusion, (a) Periods of formalin fixation, (b) Variable delay of fixation, (c) Storage of FFPE tissue blocks or sections, (d) Variable thickness of FFPE tissue sections, (e) Other variable conditions of processing FFPE tissue blocks. The stereoscopic frame of a cube represents the reliable limitation of quantitative IFIC demonstrated by serial studies as recommended in the text. Reproduced with permission from Shi et al., J. Histochem. Cytochem. 2007 55 105-109.

Shi S-R, Liu C, Taylor CR. Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval technique from experiments to hypothesis. J. Histochem. Cytochem. 2007 55 105-109. 

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