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Antigen Retrieval with Heat Treatment

Rait VK, Xu L, O Leary TJ, et al. Modeling formalin fixation and antigen retrieval with bovine pancreatic RBase A II. Interrelationship of cross-linking, immuno-reactivity, and heat treatment. Lab. Invest. 2004 84 300-306. [Pg.44]

In contrast to microwave heating, steam treatment heats slides slowly to a uniform temperature. This avoids boiling the antigen retrieval fluid and minimizes section detachment from slides. Steam heat used in combination with EDTA and protease digestion has been... [Pg.128]

This need may arise when a limited number of slides is available. For this type of study, heat-induced antigen retrieval should be carried out only once. One such treatment lasts for many months. Repeated use of antigen retrieval tends to cause background staining. Such an approach has been successfully used for staining epithelial membrane antigens and cytokeratin in the tonsil tissue fixed with Bouin s fixative (Roche et al 2000). [Pg.173]

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Antigen retrieval

Heat treatment

Retrieval

Treatment with

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