Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Microwave Heating and Ultrasound Antigen Retrieval Method

COMBINED MICROWAVE HEATING AND ULTRASOUND ANTIGEN RETRIEVAL METHOD [Pg.189]

The combined heat-induced epitope retrieval (HIER) and sonication-induced epitope retrieval (SIER) methods have been employed for retrieving cyclin Dl/bc 1-1 epitope in [Pg.189]

The slides are placed in a plastic microwavable rack and immersed in a microwavable staining dish filled with 200 ml of 0.01 M citrate buffer (pH 6.0). Blank slides are added to the rack to maintain a uniform volume in the container. The container is covered at an angle with the lid and heated twice for 5 min per cycle at 800 W in a microwave oven. Distilled water (50ml) is added after the first heating cycle to cool at room at room temperature for 10 min. [Pg.190]

The buffer (-800 ml) is heated to boiling in the microwave oven and poured into an 80-W ultrasonic cleaner (Bransonic 12, Branson Cleaning Equipment Co., Shelton, CT). The slide rack is sonicated for 1 min and then cooled in buffer in the staining container for an additional 10 min. [Pg.190]




SEARCH



And microwaves

Antigen retrieval

Antigen retrieval methods

Antigens, 1 and

HEAT method

Heating methods

Heating methods microwave

Methods microwave

Microwave heating

Microwave ultrasound

Retrieval

Retrieval methods

Ultrasound method

© 2024 chempedia.info