Antigen Retrieval in Neuronal Tissue Slices before Vibratome Sectioning

ANTIGEN RETRIEVAL IN NEURONAL TISSUE SLICES BEFORE VIBRATOME SECTIONING 

A slice 5 mm thick is cut from fixed brain tissue and washed for several hours in distilled water to remove excess formaldehyde. The slice is immersed overnight in Tris-buffered saline (TBS, pH 9.0) and then placed in a plastic jar containing 200 ml of TBS. The jar is placed in a microwave oven for 10-15 min at full power (700 W), divided into two cycles of 5 or 7.5 min to check the fluid level. The temperature is controlled using the temperature probe of the oven. It takes 3 min to reach a temperature of 90°C. 

---