Antigen retrieval techniques

RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES AND ITS APPLICATION... 

STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH... 

Shi S-R, Cote RJ, Chaiwun B, et al. Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl. Immunohistochem. 1998 6 89-96. 

Ge S, Crooks GM, McNamara G, et al. Fluorescent immunohistochemistry and in situ hybridization analysis of mouse pancreas using low-power antigen-retrieval technique. /. Histochem. Cytochem. 2006 54 843-847. 

Pileri SA, Roncador G, Ceccarelli C, et al. Antigen retrieval techniques in immunohistochemistry comparison of different methods. I. Pathol. 1997 183 116-123. 

EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION... 

Shi S-R, Gu J,Taylor CR. Antigen Retrieval Techniques Immunohistochemistry and Molecular Morphology, 1st edition. Natick, M A Eaton, 2000. 

Shi S-R, Cote RJ, Taylor CR. Antigen retrieval techniques current perspectives. I. Histochem. Cytochem. 2001 49 931-937. 

Shi S-R, Gu J, Turrens F, et al. Development of the antigen retrieval technique philosophy and theoretical base. In Antigen Retrieval Techniques Immunohistochemistry and Molecular Morphology, ed. S-R Shi, J Gu, and CR Taylor, pp. 17-39. Natick, MA Eaton, 2000. 

Shi S-R, Liu C, Taylor CR. Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval technique from experiments to hypothesis. J. Histochem. Cytochem. 2007 55 105-109. 

STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE... 

Figure 5.3 Diagram depicts the further-designed studies to test our hypothesis with respect to standardization of immunohistochemistry based on the antigen retrieval technique exemplified in a multiple direction to draw a conclusion, (a) Periods of formalin fixation, (b) Variable delay of fixation, (c) Storage of FFPE tissue blocks or sections, (d) Variable thickness of FFPE tissue sections, (e) Other variable conditions of processing FFPE tissue blocks. The stereoscopic frame of a cube represents the reliable limitation of quantitative IFIC demonstrated by serial studies as recommended in the text. Reproduced with permission from Shi et al., J. Histochem. Cytochem. 2007 55 105-109.

Shi SR, Jiang G, Turrens JF, et al. Development of the AR technique philosophical and theoretical basis. In The Antigen Retrieval Techniques Applications in Immunohistochemistry, ed. SR Shi, G Jiang, and CR Taylor. Westborough, MA BioTechniques Press, 2000. 

Although antigen retrieval techniques including HIAR continue to be regarded as enigmatic techniques, recent studies are rapidly clarifying the mechanisms of HIAR. In this article, the effects of pH and the ion concentrations of retrieval solutions on HIAR will be reviewed, and the probable mechanisms of HIAR and other antigen retrieval techniques will be discussed based on these results. 

To these facts regarding formaldehyde fixation we can then enumerate some of the things that we know about antigen retrieval techniques ... 

What Is the Optimal Way in which to use High Hydrostatic Pressures to Enhance Antigen Retrieval Techniques ... 

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