STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH

STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH 

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics, Edited by Shan-Rong Shi and Clive R. Taylor Copyright 2010 John Wiley Sons, Inc. 

TABLE 1.1 Test Battery Suggested for Screening an Optimal Antigen Retrieval Protocol 

Reprinted with permission from Shi et al.,7. Histochem. Cytochem. 1997 45 327-343. 

The increasing attention directed to the adverse effects of variation in sample preparation upon the quality of IHC staining of FFPE tissues has served to reinforce the importance of determining the optimal AR method for each antibody/detection system/antigen to achieve optimal retrieval and optimal staining of tissues that may have been processed and stored in different and unknown ways (see Chapter 5 for details). Practically, in considering 

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