Heat-induced antigen retrieval effects

BoenischT. Effect of heat-induced antigen retrieval following inconsistent formalin fixation. Appl. Immunohistochem. Mol. Morphol. 2005 13 283-286. 

Boenisch T (1999) Diluent buffer ions and pH their influence on the performance of monoclonal antibodies in immunohistochemistry. Appl Immunohistochem Mol Morphol 7 300 306 Boenisch T (2005) Effect of heat induced antigen retrieval following inconsistent formalin fixation. Appl Immunohistochem Mol Morphol 13 283 286... 

The staining protocol and differences in pretreatment methods are an important factor when formalin-fixed and paraffin-embedded material is nsed, as not all antibodies work satisfactorily under these conditions. Several different approaches can be found in the literature. Heat-induced antigen retrieval by the use of microwave heat treatment has been demonstrated to be more effective in labeling intraneuronal Ap compared to no or enzymatic pretreatment [29]. Information on the use of FA is inconsistent. It is widely used to increase the immunoreactiv-ity of amyloid plaques in AD however, it has been shown that the effect on intraneuronal Ap is low and that use of FA sometimes results in a loss of cellular detail [30]. A recent study tried to quantify the effect of FA pretreatment and analyzed plaque load and intraneuronal Ap immunoreactivity using an APi, antibody in 6-month-old APP/PSIKI mice (Fig. 3 reproduced from [25] with permission from Elsevier). 

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