Glycerin as Antigen Retrieval Fluid

Alternatively, such high temperatures in the bowl can be achieved by placing it in a microwave oven, then removing and placing it on the hot plate. This is followed by adding the stir bar and the slide rack to the bowl. It takes 1 min for 100 ml of the glycerin solution to attain a temperature of 125°C in a 600 W microwave oven on high. If more than 100 ml of solution is to be heated to the same temperature, for every additional 100 ml an additional 1 min is required. 

Generally Tris-HCl buffer produces better results at higher pH levels (e.g., pH 10.0) than do some other buffers. On the other hand, sodium citrate buffer increases the intensity and extent of immunostaining of a wide variety of tissue antigens at pH 6.0. EDTA-NaOH (1 mM) at pH 8.0 also yields satisfying results. Although relatively high pH solutions, such 

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