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Antigen Retrieval Methods A Summary

The following recent comparative studies demonstrate that no single antigen retrieval method is optimal for all types of antigens. [Pg.153]

Immunostaining of Aa and A (3 isoforms of calcineurin in the human brain employing CAN-2 and CAN-3, respectively, was compared between microwave heating (in 10 mM sodium citrate at pH 6.0 for 10 min) and autoclaving at 120°C for 20 min (Usuda et al., 1996). The former approach was the most effective for intensification of the immunoreaction. [Pg.153]

Compared to enzyme digestion methods, microwave heating demonstrated more intense immunoreactivity of estrogen and progesterone in breast cancer tissues fixed with methacam (60% methanol, 30% chloroform, and 10% acetic acid) (Oyaizu et al., 1996). [Pg.153]

Compared with trypsin digestion, microwave heating produced more consistent results and was effective over a greater range of fixation tissues in the case of immunoglobulin light chain in tonsil tissue (Ashton-Key et al., 1996). [Pg.153]

Compared with pepsin predigestion, microwave heating markedly enhanced the staining of aberrant p53 antigen with Pab 1801-D07 antibody cocktail in paraffin or frozen sections in adenocarcinoma of the lung (Resnick et al., 1995). [Pg.153]


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