Antigen Retrieval on Sections of Modified Epoxy Resin

ANTIGEN RETRIEVAL ON SECTIONS OF MODIFIED EPOXY RESIN 

The sections are treated with 10% BSA in PBS (pH 7.2) for 4 hr to block nonspecific labeling. Incubation is carried out overnight at 4°C in the primary antibody, appropriately diluted in PBS. This is followed by washing three times for 5 min each in PBS and incubation for Dhr at 22°C in colloidal gold (15 nm)-conjugated secondary antibody, appropriately diluted in PBS containing 3% BSA. The sections are poststained with 5% uranyl 

FIGURE 7.3. Schematic illustration of increased exposure of epitopes on the section surface as a result of heating the sections in citrate buffer. Reprinted, with permission, from Brorson (1998a). Copyright 1998 Elsevier Science. 

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