High temperature antigen retrieval

Slater MD, Murphy CR. Co-expression of interleukin-6 and human growth hormone in apparently normal prostate biopsies that ultimately progress to prostate cancer using low pH, high temperature antigen retrieval. /. Mol. Histol. 2006 37 37-41. 

Morgan JM, Navabi H, Schmid KW, et al. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. /. Pathol. 1994 174 301-307. 

Morgan JM, Navabi H, Jasani B. Role of calcium chelation in high-temperature antigen retrieval at different pH values. J. Pathol. 1997 182 233-237. 

When using high-temperature antigen retrieval, you may skip blocking alkaline phosphatase, since some endogenous enzymes, such as alkaline and acid phosphatases, in contrast to peroxidase, are destroyed by boiling for even a short time at 100°C (Cattoretti et al. 1993). 

Morgan, J. M., Jasani, B., and Navabi, H. (1997) A mechanism for high temperature antigen retrieval involving calcium complexes produced by formalin fixation. J. Cell. Pathol. 2, 89-92. 

Grabau, D. A., Nielsen, O., Hansen, S., Nielsen, M. M., Laenkholm, A.-V., Knoop, A., and Pfeiffer, P. 1998. Influence of storage temperature and high temperature antigen retrieval buffers on results of immunohistochemical staining in sections stored for long periods. Appl. Immunohistochem. 6 209-213. 

Hunt, N. C. A., Attanoos, R., and Jasani, B. 1996. High temperature antigen retrieval and loss of nuclear morphology A comparison of microwave and autoclave techniques. J. Clin. Pathol. 49-101-110. 

Grabau KA, Nielsen O, Hansen S. Influence of storage temperature and high-temperature antigen retrieval buffers on results of immunobistochemical staining in sections stored for long periods. Appl Immunohistochem. 1998 6 209. 

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