Turbulent-flow liquid chromatography

The most common (off-line) sample preparation procedures after protein precipitation are solid phase extraction and liquid-liquid extraction. Multiple vendors and available chemistries utilize 96-well plates for solid phase extraction systems and liquid-liquid extraction procedures. Both extraction process can prepare samples for HPLC/MS/MS assay. Jemal et al.110 compared liquid-liquid extraction in a 96-well plate to semi-automated solid phase extraction in a 96-well plate for a carboxylic acid containing analyte in a human plasma matrix and reported that both clean-up procedures worked well. Yang et al.111 112 described two validated methods for compounds in plasma using semi-automated 96-well plate solid phase extraction procedures. Zimmer et al.113 compared solid phase extraction and liquid-liquid extraction to a turbulent flow chromatography clean-up for two test compounds in plasma all three clean-up approaches led to HPLC/MS/MS assays that met GLP requirements. [Pg.212]

Asperger A. et al., 2002. Trace determination of priority pesticide in water by means of high-speed online solid-phase extraction-liquid chromatography-tandem mass spectrometry using turbulent-flow chromatography columns for enrichment and a short monolithic column for fast liquid chromatographic separation. J Chromatogr A 960 109. [Pg.293]

Grant R.P., Cameron C., and Mackenzie-McMurter S., 2002. Generic serial and parallel online direct injection using turbulent flow liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 16 1785. [Pg.294]

Xu X. et al., 2005. Quantitative determination of a novel dual PPAR aly agonist using online turbulent flow extraction with liquid chromatography-tandem mass spectrometry. J Chromatogr B 814 29. [Pg.297]

Ynddal L. and Hansen S.H., 2003. Online turbulent-flow chromatography-high-performance liquid chromatog-raphy-mass spectrometry for fast sample preparation and quantitation. J Chromatogr A 1020 59. [Pg.297]

Zimmer, D., Pickard, V., Czembor, W. and Muller, C. (1999) Comparison of turbulent-flow chromatography with automated solid-phase extraction in 96-well plates and liquid-liquid extraction used as plasma sample preparation techniques for liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 854 (1-2), 23-35. [Pg.239]

Takino, M., Daishima, S., and Nakahara, T. (2003). Determination of perfluorooctane sulfonate in river water by liquid chromatography/atmospheric pressure photoionization mass spectrometry by automated on-line extraction using turbulent flow chromatography. Rapid Commun. Mass Spectrom. 17, 383—390. [Pg.506]

The term A is related to the flow profile of the mobile phase as it traverses the stationary phase. The size of the stationary phase particles, their dimensional distribution, and the uniformity of the packing are responsible for a preferential path and add mainly to the improper exchange of solute between the two phases. This phenomenon is the result of Eddy diffusion or turbulent diffusion, considered to be non-important in liquid chromatography or absent by definition in capillary columns, and WCOT (wall coated open tubular) in gas phase chromatography (Golay s equation without term A, cf. 2.5). [Pg.18]

In most common turbulent-flow chromatography experiments, a second conventional high-performance liquid chromatography (HPLC) column is employed. The conventional HPLC column, usually referred to as the analytical column, is used for peak sharpening and additional separation prior to MS detection. This approach requires an additional valve and pump to control the final HPLC separation. A typical dual-column methodology is shown in Fig. 10.5. [Pg.319]

Martin, M., and Guiochon, G. (1982). Influence of retention on band broadening in turbulent-flow liquid and gas chromatography. Anal. Chem. 54 1533-1540. [Pg.338]

Xu, X., Yana, K. X., Songa, H., and Loa, M. W. (2005a). Quantitative determination of a novel dual PPAR a/y agonist using on-line turbulent-flow extraction with liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 814 29-36. [Pg.339]

Zhou, S., Zhou, H., Larson, M., Miller, D. L., Mao, D., Jiang, X., and Naidong, W. (2005). High-throughput biological sample analysis using on-line turbulent-flow extraction combined with monolithic column liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 19 2144-2150. [Pg.340]

Sauvage FL, Gaulier JM, Lachatre G et al (2006) A fully automated turbulent-flow liquid chromatography-tandem mass spectrometry technique for monitoring antidepressants in human serum. Ther Drug Monit 28 123-130... [Pg.173]

Breaud AR, Harlan R, Di Bussolo JM et al (2010) A rapid and fully-automated method for the quantitation of tricyclic antidepressants in serum using turbulent-flow liquid chromatography-tandem mass spectrometry. Clin Chim Acta 411 825-832... [Pg.174]

Ynddal, L. Hansen, S.H. On-Line Turbulent-Flow Chromatography-High-Performance Liquid Chromatography-Mass Spectrometry for Fast Sample Preparation and Quantitation, J. Chromatogr. A 1020(1), 59-67 (2003). [Pg.187]

Grant, R.P Cameron, C. Mackenzle-McMurter S. "Generic Serial and Pai allel On-line Direct-injection Using Turbulent Flow Liquid Chromatography/Tandem Mass Spectrometry, Rapid Commun. Mass Spectrom. 16, 1785— 1792 (2002). [Pg.225]

FIGURE 11.1 A generalized flow scheme that indicates the fundamental elements of LC-MS-based bioanalysis. Abbreviations LLE = liquid-liquid extraction SPE = solid-phase extraction RAM = restricted-access media TEC = turbulent flow liquid chromatography API = atmospheric-pressure ionization APCI = atmospheric-pressure chemical ionization ESI = electrospray ionization SQMS = single-quadrupole mass spectrometry TQMS = triple-quadrupole mass spectrometry TOF = time-of-flight Q-TOF = quadrupole TOF. (Reprinted from Ackermann et al. [4], with permission from John Wiley Sons, Inc.)... [Pg.318]

Zimmer, D. Pickard, V. Czembor, W. Muller, C. Comparison of Turbulent Flow Chromatography with Automated Solid-Phase Extraction in 96-Well Plates and Liquid-Liquid Extraction used as Plasma Separation Techniques for Liquid Chromatography-Tandem Mass Spectrometry, J. Chromatogr. A 854, 23-35 (1999). [Pg.505]

Many different sample preparation techniques are available to the drug discovery scientist. Off-line sample preparation procedures include protein precipitation, filtration, dilution followed by injection, liquid-liquid extraction (LLE), and solid-phase extraction (SPE). Typically, these procedures are performed in an automated, high-throughput mode that features a 96-well plate format. Online sample preparation procedures include SPE and turbulent flow chromatography (TFC) with conventional chromatographic media or restricted access media (RAM). These online approaches are often simple and easy to automate. [Pg.43]