Single quadrupole mass

G. J. Opiteck, J. W. Jorgenson, M. A. Moseley III and R. J. Anderegg, Two-dimensional mia ocolumn HPLC coupled to a single-quadrupole mass spectrometer for the elucidation of sequence tags and peptide mapping , 7. Microcolumn Sep. 10 365-375 (1998). 

Voyskner, R. D. Pack, T. Investigation of collisional-activation decomposition process and spectra in the transport oregion of an electrospray single-quadrupole mass-spectrometer. Rapid Comm. Mass Spectrom. 1991, 5, 263-268. 

Recently, comparatively inexpensive, very reliable, and stable single quadrupole mass spectrometers have entered the market. These spectrometers can be coupled to GC, LC, and CE separation methods simply by modifying the sampling interfaces. Although these detectors are more expensive than most conventional detectors including the versatile electron capture and diode array absorbance detectors used for GC and LG respectively, the reduction in sample preparation effort and their increased specificity can often rapidly... 

Fig. 1.6 (A) Electron impact spectrum obtained on a single quadrupole mass spectrometer of a compound with Mr = 355. (B) Product ion spectrum after atmospheric pressure ionization obtained on a triple quadrupole instrument. Chemical ionization and atmospheric pressure ionization give in both cases protonated precursor ions, which is ideal for tandem mass spectrometry.

Mullen, W., Lean, M.E.J., and Crozier, A., Rapid characterization of anthocyanins in red raspberry fruit by high-performance liquid chromatography coupled to single quadrupole mass spectrometry, J. Chromatogr. A, 966, 63, 2002. 

V. Katta, S. K. Chowdhury, and B. T. Chait, Use of a single-quadrupole mass spectrometer for collision-induced dissociation studies of multiply charged peptide ions produced by electrospray ionization, Anal. Chem., 63 (1991) 174-178. 

Detection was performed on a single quadrupole mass spectrometer by SIM mode via APCI source. The intra- and interday precisions were... 

Detection of imatinib is performed by triple quadrupole mass spectrometer with an electrospray ionization (ESI) interface operated in positive ion mode [103, 104, 106,107,109,110]. Except the methods published by Parise et al. for imatinib and its main metabolite [108], and for nilotinib [111], where a single quadrupole mass spectrometer was used, most TKIs are analyzed in plasma by atmospheric pressure ionization (electrospray or turbo ion spray) coupled to triple stage mass spectrometer. Expectedly, higher limit of quantifications for imatinib (30 ng/ml) [108], and nilotinib (5 ng/ml) [111], are obtained for the assays using single quadrupole MS (see Table 2). 

Lately, the development of an assay by LC coupled to single quadrupole mass spectrometry has been recently published for the determination of cellular concentrations of imatinib, dasatinib, and nilotinib in PBMCs, but the authors did not give much details on the results obtained with patients samples [144],... 

For abbreviation of analyte names see Sect. Abbreviations . ACN acetonitrile, APCI atmospheric pressure chemical ionization, dial, microdialysis samples, ESI electrospray ionization, FA formic acid, iso isocratic, IT ion trap, lin range linear range, MeOH methanol, MRM multiple reaction monitoring, MS full scan mass spectrometry, n.s. not specified, OAc acetate, QqQ triple quadrupole mass spectrometer, SIM selected ion monitoring, Solv HPLC solvent, SQ single quadrupole mass spectrometer, T temperature Ratios given as v/v... 

Besides the triple quadrupol instruments, other types of mass spectrometers might be used as well. Examples for these types of instruments are ion traps, time of flight mass spectrometers and also single quadrupol mass analyzers. Due to the characteristic and specific advantages and disadvantages of different instrument types, the overall assay performance (e.g. sensitivity, dynamic range and selectivity) may vary quite a bit from one instrument type to the other. 

The solvent gradient delivery is via a HP 1050 LC system equipped with a 100 position extended tray and a HP Diode-array detector integrated and controlled by a single-quadrupole mass spectrometer running MassLynx on Windows NT. The system is accessed via a separate Login PC through OpenLynx (Fig. 5.5). 

Fig. 8 A separation from an open-tubular column monitored by a single quadrupole mass spectrometer.

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