Biological high throughput

Zhou S. et al., 2005. High-throughput biological sample analysis using online turbulent flow extraction combined with monothic column liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 19 2150. 

Daniele N, Halse R, Grinyo E, Yeaman SJ, Shepherd PR. Conditionally immortalized cell lines as model systems for high-throughput biology in drug discovery. Biochem Soc Trans 30 800-802 (2002). 

Multiple Parallel Synthesis (MPS) is typically carried out in a plate containing a matrix of 8 x 12 wells. It is typical for each well to contain a different combination of reactants (say an acid and an amine). Since the number of outcomes is then restricted (to say 96 amides) it could reasonably be argued that a full analysis of individual spectra need not take place in order to validate the plate for further work. Indeed, it may well be that the plate is destined for high throughput (biological activity) screening, and only hits from that process would then warrant a more rigorous approach. 

Zhou, S., Zhou, H., Larson, M., Miller, D. L., Mao, D., Jiang, X., and Naidong, W. (2005). High-throughput biological sample analysis using on-line turbulent-flow extraction combined with monolithic column liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 19 2144-2150. 

A more random approach to discovering a lead is the combinatorial chemistry approach (see Chapter 6). This uses a simultaneous multiple synthesis technique to produce large numbers of potential leads. These potential leads are subjected to rapid high throughput biological screening to identify the most active lead compounds. Once identified, these lead compounds are subject to further development. 

Predictive models for anti-tubercular molecules using machine learning on high-throughput biological screening datasets. BMC Res Notes 4 504... 

Stephen G. Withers (B.Sc. and Ph.D.) was trained at the University of Bristol, UK, where he obtained his Ph.D. under the supervision of Dr. Michael Sinnott. Later he moved to Canada as a postdoctoral fellow, applying heteronuclear NMR to the study of enzymatic catalysis with Drs. Brian Sykes and Neil Madsen in the Department of Biochemistry at the University of Alberta. In 1982 he moved to the University of British Columbia as assistant professor of chemistry. He now holds the Khorana Chair of Chemistry and Biochemistry at UBC and serves as the director of CHiBi, the Centre for High-throughput Biology at UBC. 

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