Human HeLa cancer cells

Mlerenes at 6 pM and white light to produce growth inhibition in human HeLa cancer cells. However, these same authors later reported that other carboxylic acid derivatives of C60 and C70 were completely without any photoactivity as PDT agents at 50pM (Irie et al., 1996). 

In 1999, Rickards et al. reported the isolation of calothrixins A (377) and B (378) from photoautrophic cultures of Calothrix cyanobacteria (345). These two, novel, pentacyclic carbazole alkaloids contain a quinolino[4,3-fc]carbazole-l,4-quinone framework. Calothrixins A and B inhibit the growth of a chloroquin-resistant strain of the malaria parasite P. falciparum and human HeLa cancer cells (345). 

The test system was considerably less sensitive to endosulfan when mouse ER, rather than human ER, was used to mediate (3-gal activity (Ramamoorthy et al. 1997). In similar assays, endosulfan at 10 jM had no effect on (3-gal activity in yeast Saccharomyces) transfected with either the human or rainbow trout ER (Andersen et al. 1999). In addition, no effect was observed on transcriptional activation of HeLa cells transfected with plasmids containing an estrogen receptor as a responsive element (Shelby et al. 1996). Endosulfan also did not induce transient reporter gene expression in MCF-7 human breast cancer cells at an incubation concentration of 2.5 pM (Andersen et al. 1999). Maximum endosulfan-induced ER-mediated luciferase reporter gene expression occurred in vitro in a T47D human breast adenocarcinoma cell line at approximately 10 pM, while 50% expression of luciferase occurred at about 5.9 pM the maximum expression was approximately 59% of the effect from exposure to 0.03 nM estradiol (0.00003 pM) (Legler et al. 1999). Luciferase expression from combined treatment with endosulfan and dieldrin was additive over concentrations ranging from 3 to 8 pM. 

Cell cycle redistribution may not be the sole factor if cells are irradiated before drug exposure, but it has been shown that 5-FU can sensitize even when cells are irradiated before drug exposure. Byfield et al. found that 5-FU radiosensitizes HeLa cells only when the drug exposure followed radiation (the cells were treated with 5-FU, either before or after radiation, for up to 8 d). A similar finding was observed on HT29 human colon cancer cells, except that in these experiments cells were exposed to 5-FU for a maximum of only 30 min before radiation (33). These observations demonstrate that radiosensitization can be produced in the absence of cell cycle redistribution. 

FIGURE 8.8 A section of a single HeLa (human cervical cancer) cell stained with Phalloidin 350 (actin filaments) and MitoTracker488 (mitochondria) acquired using selective two-photon excitation, without fluorescence color filters. The image size is about 25 (xm. (From Dantus, M., Lozovoy, V. V., and Pastirk, I. Laser Focus World, 43(5) 101-104. 2007. Used with permission.)... 

EAG has a rather restricted distribution in healthy tissues it expresses nearly exclusively in brain, slightly in placenta and transiently in skeletal muscle (Occhiodoro et al. 1998 Pardo et al. 1999). In contrast to its restricted distribution in normal tissues, expression of EAG has been found in several human somatic cancer cell lines including HeLa cervix carcinoma, MCF-7 and SHSY-5Y neuroblastoma derived from breast tumor, melanoma IGR1, rhabdomyosarcoma, etc. (Meyer 1998, 1999 Crociani 2003). Most notably, EAG mRNA expression was found in 100% of the human cervical cancer sam-... 

Butter yellow is active in inducing unscheduled DNA synthesis in Hela human cervical cancer cells. It was mutagenic in Salmonella typhimurium TAIOO and TA98. 

Zacharewski et al. (1998) found that di- -butyl phthalate induced reporter gene (luciferase) activity in MCF-7 human breast cancer cells, but not HeLa human cervical carcinoma cells, transfected with human estrogen receptor, although approximately 3,000-fold less potent than 17p-estradiol. Additionally, Zacharewski et al. (1998) found that di- -butyl phthalate was capable of supporting a very modest amount... 

HeLa Human cervical cancer cell line... 

Anonaine AT m dissipation, increase of intracellular nitric oxide, ROS, glutathione depletion, caspase activation and PARP cleavage Human cervical cancer cell Hela Alkaloid isolated from plant M c/ze/ifl alba (Magnoliaceae) [129]... 

Using the same procedure from mannose, mannosylamine was coupled with cyclic AT-acetylpenicillamine to afford after nitrosation a gly-coconjugate with potent cytotoxicity against DU 145 human prostate cancer cells and hela R cancer cells (Scheme 13). ... 

GA exhibits a ROS-mediated anticancer activity in human prostate cancer cells [63] and possesses antimelanogenic properties. A phenolic fraction from evening primrose Oenothera biennis) containing 55% of GA showed antitumor activity [64]. It has been proposed as a candidate for treatment of brain tumors as it suppresses cell viability, proliferation, invasion, and angiogenesis in human glioma cells. GA induces the death of HeLa cervical cancer cells via apoptosis and/or necrosis [17]. 

Pan et al. reported Tan-IIA exhibited strong growth inhibition against human cervical cancer cells (HeLa cells) in dose- and time-dependent manners with a 50% ceU growth inhibition with 2.5 pg/mL (8.49 pM). The authors executed a bioinformatics analysis of proteins to survey global protein changes induced by Tan-IIA... 

K562 myelogenous leukaemia cells, A549 carcinomic human alveolar basal epithehal cells, BGC-823 human gastric carcinoma cell, CNE human nasopharyngeal carcinoma cell, HeLa human cervical cancer cells... 

The volume of work on the antitumor activity and cytotoxicity of ILs and ILBSs is limited, with more publications on the effects ILs than their surface-active counterparts. Although discussion on the former class of compounds is outside the scope of the present article, we note that imidazolium-, phosphonium-, and ammonium-based ILs have been employed against 60 human tumor cell lines, including brain tumor cells, [83], human hepatocellular liver carcinoma [84], HeLa cancer cells [85], and human melanoma cell lines [86],... 

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