Human breast adenocarcinoma

The test system was considerably less sensitive to endosulfan when mouse ER, rather than human ER, was used to mediate (3-gal activity (Ramamoorthy et al. 1997). In similar assays, endosulfan at 10 jM had no effect on (3-gal activity in yeast Saccharomyces) transfected with either the human or rainbow trout ER (Andersen et al. 1999). In addition, no effect was observed on transcriptional activation of HeLa cells transfected with plasmids containing an estrogen receptor as a responsive element (Shelby et al. 1996). Endosulfan also did not induce transient reporter gene expression in MCF-7 human breast cancer cells at an incubation concentration of 2.5 pM (Andersen et al. 1999). Maximum endosulfan-induced ER-mediated luciferase reporter gene expression occurred in vitro in a T47D human breast adenocarcinoma cell line at approximately 10 pM, while 50% expression of luciferase occurred at about 5.9 pM the maximum expression was approximately 59% of the effect from exposure to 0.03 nM estradiol (0.00003 pM) (Legler et al. 1999). Luciferase expression from combined treatment with endosulfan and dieldrin was additive over concentrations ranging from 3 to 8 pM. 

Goldberg-Bittman L, Neumark E, Sagi-Assif O, et al. The expression of the chemokine receptor CXCR3 and its ligand, CXCL10, in human breast adenocarcinoma cell lines. Immunol Lett 2004 92 171-178. 

Dasgupta P, Singh A, Mukherjee R (2002) N-terminal acylation of somatostatin analog with long chain fatty acids enhances its stability and anti-proliferative activity in human breast adenocarcinoma cells. Biol Pharm Bull 25 29-36 Delgado C, Francis GE, D. F (1992) The uses and properties of PEG-linked proteins. Crit Rev Ther Drug Carrier Syst 9 249-304... 

Moongkarndi, P., Kosem, N., Luanratana, O., Jongsomboonkusol, S. and Pongpan, N. (2004b) Antiproliferative activity of Thai medicinal plant extracts on human breast adenocarcinoma cell line. Fitoterapia 75(3—4), 375-377. 

Cell line expressing oncogenic KRAS, Cell lines A549, human lung adenocarcinoma CaLu-1, human nonsmaU cell lung cancer MDA-MB-231, human breast cancer PANC-1, human pancreatic cancer MMTV, mouse mammary tumor virus n/d, not determined p-, phosphorylated form of a protein. 

Leveque J, Eoucher E, Havouis R, Desury D, Grail J-Y, Mouli-noux J-P. Benefits of complete polyamine deprivation in hormone responsive and hormone resistant MCF-7 human breast adenocarcinoma in vivo. Anticancer Res. 2000 20(1A) 97-101. 

Noel, A., de Pauw-Gillet, M. C., Purnell, G., Nusgens, B., Lapiere, C. M. and Foidart, J. M. (1993). Enhancement of tumorigenicity of human breast adenocarcinoma cells in nude mice by matrigel and fibroblasts. Br. J. Cancer 68, 909-915. 

Human breast adenocarcinoma MCF-7 cells were incubated for different time intervals with deuterated LIP and TATp-LIP at a Upid concentration of 2 mg ml" . After incubation, the cells were fixed in a phosphate-buffered formalin solution and subsequently washed and submerged in phosphate-buffered saline, to maintain the cell shape within the aqueous environment. 

Kramer VJ, Helferich WG, Bergman A, et al. 1997. Hydroxylated polychlorinated biphenyl metabolites are anti-estrogenic in a stably transfected human breast adenocarcinoma (MCF7) cell line. Toxicol Appl Pharmacol 144 363-374. 

We confirmed that this assay could be used to quantify active caspase-3 and active caspase-7 in other cell types. Active caspase-3 and active caspase-7 were quantified in the extracts from STS-treated human neuroblastoma (CHP-100 and SH-SY5Y) and promyelocytic leukemia (HL60) cells (Table 1). The amount of active caspase-3 was found to be much higher than the amount of active caspase-7 in these cell types. Very low or no amounts of active caspases were detected in control cells (cells not treated with STS). Active caspase-3 and active caspase-7 were also quantified in human breast adenocarcinoma (MCF-7) cells that do not express caspase-3 (28,38). No... 

Note A-549 human lung carcinoma MCE-7, human breast carcinoma HT-29, human colon adenocarcinoma NSCLCN6, human bronchial epidermoid carcinoma KB, nas opharyngeal... 

Dr Keisuke Iwamoto used genetically-modified human breast adenocarcinoma cell line (MCF-7) cells to overexpress sodium iodide symporter (NIS) and/or LPO, to test the hypothesis that the survival advantage conferred by iodide to human breast cancer cells requires the suppression of LPO, i.e., iodide, but not oxidized iodide (I2) (Iwamoto and Kim, 2005). The model used ionizing radiation (IR)... 

---